摘要
枯草杆菌中性蛋白酶基因在大肠杆菌中的表达刘白玲何先祺（四川联合大学皮革工程系成都６１００６５）张义正（四川联合大学生物工程系成都６１００６４）蛋白酶是枯草杆菌（Ｂａｃｉｌｕｓｓｕｂｔｉｌｉｓ）产生的具有重要工业价值的水解酶。对蛋白酶基因的分离与高效...
An Escherichia coli Bacillus subtilis shuttle vector pNPR10 containing a neutral protease(nprE) gene of B.subtilis was used to transform the E.coli DH5αF′ and E.coli JM107 for study of the expression of npr gene of B.subtilis in E.coli and its expression level. The purpose of the research was to develop a method of screening npr gene with E.coli as a host. The results indicated that the intact npr gene of B.subtilis could be expressed in E.coli and the expressed neutral protease was mainly distributed in the cytoplasm and partially in periplasmic space of E.coli . The E.coli could be the host for the isolation of npr gene if the colony lysis in situ was used.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第3期304-308,共5页
Chinese Journal of Biotechnology
基金
国家教委年轻教师基金
关键词
基因表达
蛋白酶基因
枯草杆菌
大肠杆菌
Bacillus subtilis, Escherichia coli , neutral protease gene, gene cloning, gene expression
