摘要
目的：研究增殖细胞核抗原（ＰＣＮＡ）及Ｃ－ｍｙｃ基因过度表达在子宫颈上皮内瘤变（ＣＩＮ）及子宫颈鳞癌中的变化及其临床意义。方法：用免疫组化方法检测ＣＩＮ及子宫颈鳞癌手术标本石蜡包埋切片中的ＰＣＮＡ及Ｃ－ｍｙｃ基因蛋白变化并分析子宫颈癌临床病理资料。结果：从正常子宫颈、ＣＩＮ到子宫颈鳞癌，ＰＣＮＡ含量及Ｃ－ｍｙｃ阳性表达呈增高趋势。ＣＩＮⅢ及子宫颈鳞癌的ＰＣＮＡ标记指数（ＰＣＮＡＬＩ）及Ｃ－ｍｙｃ阳性表达明显高于正常子宫颈及ＣＩＮⅠ－Ⅱ，并且Ｃ－ｍｙｃ基因阳性表达与ＰＣＮＡ大量出现呈正相关。ＰＣＮＡＬＩ及Ｃ－ｍｙｃ阳性表达随细胞分化程度而增加。子宫颈鳞癌分期及有无淋巴结转移对ＰＣ－ＮＡＬＩ及Ｃ－ｍｙｃ阳性表达差异无显著性。结论：检测ＣＩＮ及子宫颈鳞癌中ＰＣＮＡ数量及Ｃ－ｍｙｃ基因表达可了解宫颈癌的发生、发展，早期发现癌前病变及估计肿瘤的恶性潜能。
)Objective To study the changes of PCNA and C myc oncogene overexpression and it's clinical value in CIN and cervical squamous carcinoma. Methods Immunohistochemical staining was used to detect PCNA and C myc oncogene overexpression products in CIN and cervical squamous carcinoma tissues obtained from 42 patients which were operated in Obstet and Gynecol Hospital.Clinical and pathological materials were analyzed. Results The PCNA LI and C myc positive expression were increased gradually from normal cervical epithelium,CIN to cervical squamous carcinoma.The PCNA LI and C myc positive expressions of CINⅢ and cervical squamous carcinoma were conspicuously higher than that of normal cervical epithelium and CINⅠ Ⅱ.C myc positive expression was positively correlated with high PCNA LI.PCNA LI and C myc positive expression increased with increasing pathological grade.There was no relationship among PCNA LI,C myc positive expression and clinical staging,lymph node metastases of cervical squamous carcinoma. Conclusion To detect the changes of oncogene expression in CIN and cervical squamous carcinoma could understand the development of cervical carcinoma,early determination of premalignancies and evaluation of malignant potential of tumor tissues.
出处
《现代妇产科进展》
CSCD
1997年第3期204-207,共4页
Progress in Obstetrics and Gynecology
关键词
子宫颈肿瘤
C-MYC基因
上皮内癌变
鳞癌
PCNA
Proliferating cell nuclear antigen
C myc oncogene
Cervical intraepithelial neoplasia
Cervical squamous carcinoma.
