摘要
[目的]检测杠柳毒苷在大鼠和人体肠菌作用下含量与成分的变化。[方法]采用离体粪便温孵法,利用高效液相—紫外法对温孵不同时间的杠柳毒苷及其代谢产物含量进行测定,并利用高效液相—质谱联用法对代谢物进行鉴定。[结果]杠柳毒苷含量随温孵时间的延长呈快速下降的趋势,温孵5h含量降低约75%,与此同时有代谢物生成,并随温孵时间的延长呈上升趋势。[结论]杠柳毒苷在肠菌(大鼠/人体)作用下,代谢迅速,生成了代谢产物杠柳次苷。
[Objective] To investigate the change of content and component of periplocin in Vitro under the action of intestinal flora in rat and human. [Methods] Periplocin was incubated with intestinal flora from rat and human. HPLC-UV method was used to quantitate the content of Periplocin and their metabolites. HPLC-MS method was used to qualitate the metabolite. [Results] The content of periplocin decreased fast after incubation with intestinal flora. It decreased about 75% after incubation for 5 hours. At the same time, a metabolite appeared, It＇ s content raised and related with the time of incubation. [Conclusions] Periplocin can be disintegrated fastly into periplocymatin by intestinal bacterium.
出处
《天津中医药》
CAS
2007年第6期515-518,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
天津市高等学校科技发展基金项目(20040303)
天津市科技发展计划项目(05YFJZJC01102)。
关键词
杠柳毒苷
杠柳次苷
强心苷
肠菌代谢
粪便温孵
代谢转化
periplocin
periplocymarin
cardiac glycoside
metabolism by intestinal bacterium
incubation with intestinal flora
metabolism conversion
