摘要
为研究瘤胃微生物对瘤胃液中添加的蛋氨酸、赖氨酸的利用情况,并进一步探讨添加氨基酸对瘤胃液中尿素氮、部分酶活的影响,将采集到的来自3头装有永久性瘤胃瘘管的成年萨能山羊的瘤胃液混合后,平均分装成18个瓶,随机分成3个处理组,每组6个重复。采用6重复随机因子试验设计。第1组中每瓶添加蛋氨酸2mM(0.25mol/LMet溶液8mL),第2组中每瓶添加赖氨酸2mM(0.25mol/LLys溶液8mL),第3组中每瓶加等体积蒸馏水作为空白对照组,体外培养16h。结果表明:添加氨基酸后,混合瘤胃液中的尿素氮(UN)含量显著高于对照组(P<0.05);谷氨酸脱氢酶(GLDH)活性随着体外培养时间的延长而迅速增加,并且添加蛋氨酸组的GLDH的活性明显高于其他两组(P<0.01),而添加赖氨酸组的GLDH活性则极显著低于蛋氨酸组和对照组(P<0.01);添加的氨基酸和体外培养时间对于谷丙转氨酶(GPT)活性没有显著的影响(P>0.05);添加氨基酸组的谷草转氨酶(GOT)的活性显著低于对照组(P<0.05),但体外培养时间对各组中GOT的活性没有显著的影响。说明体外培养过程中瘤胃微生物分解赖氨酸的速率要比蛋氨酸快;添加的氨基酸使UN增加,但对GOT、GPT的活性起抑制作用;添加蛋氨酸可增加GLDH活性,但添加赖氨酸却有抑制作用。
The aim of this study was to investigate the utilization of supplementary methionine and lysine by mixed rumen microorganisms in vitro ,and its further effects on urea nitrogen （UN） and enzyme activities （glutamic pyruvic transaminase GPT, glutamic oxalacetic transaminase GOT and glutamic acid dehydrogenase GLDH）. Rumen fluids from three perpetually fistulated Salon goats were collected and Mixed, then divided into 18 bottles and allocated into three treatments arranged in a randomized complete block design with 6 with adding methionine（2mM）, lysine（2mM） and equal distilled water as control groups, at last all treatments incubated for 16 hours at 39℃ in vitro. Results indicated that the content of the UN in mixed rumen fluid increased when adding methionine or lysine compared with that in control group （P〈0. 05）. The activity of the GLDH increased rapidly with culture time. DLDH activity in methionine group was extremdy higher than that in other two groups（P〈0. 01 ）, however, DLDH activity in lysine group was extremdy lower than that in other two groups（P〈0. 01 ）. Culture time and kind of amino acids had no effect on GPT activity. GOT activities in both methionine and lysine groups were significantly lower than that in control（P〈0. 05）, and culture time had no significant influence on them. Lysine was degraded more quickly than the methionine by rumen microorganisms during culture in vitro. The UN increased when adding amino acids, however activities of GOT and GPT were inhibited, and activities of GLDH increased when adding methionine.
出处
《中国草食动物》
CAS
2007年第6期9-11,共3页
China Herbivores
基金
安徽省"十五"攻关项目(040030343)
合肥市重点农业科研项目(2005-1011)
