摘要
牙鲆、鲈鱼和真鲷的四个永生性细胞系建立童裳亮李宏＊＊苗宏志（青岛海洋大学生命学院，青岛２６６００３）牙鲆（Ｐａｒａｌｉｃｈｔｈｙｓｏｌｉｖａｃｅｕｓ）、鲈鱼（Ｌａｔｅ－ｏｌａｂｒａｘｊａｐｏｎｉｃｕｓ）和真鲷（Ｐａｇｒｏｓｏｍｕｓｍａｊｏｒ）是我国沿...
The established four continuous marine fish cell lines are the flounder ( Paralichthys olivaceus ) gill cell line FG at the passage number 105,the sea perch ( Lateolabrax japonicus ) spleen and heart cell lines SPS and SPH at the passage numbers 67 and 80,and the red sea bream ( Pagrosomus major ) fin cell line RSBF at the passage number 62.The three species of fish are widely cultured in China.Diseases occurred frequently in the recent years due to the intensive culture.The establishment of the cell lines are essential for monitoring the viral and rickettsial diseases of marine fish.All of the cell lines had the similar growth responses to temperatures.The best growth occurred in the temperature range of 20-25℃.At 15℃ the cell grew very slowly but could survive for one or two months without medium replacement.The chromosome counts showed the occurrence of aneuploidy and heteroploidy which suggested that the cells were transformed.
出处
《生物工程进展》
CSCD
1997年第3期3-4,共2页
Progress in Biotechnology
基金
国家自然科学基金
