摘要
果胶细菌处理油菜幼根（2天苗龄、根长3cm），同时接种紫云英根瘤菌突变菌株（Rhizobiumhuakuii），诱发类根瘤的结瘤率为96.80％。从这些类根瘤中分离的菌株能回接到原寄主。类根瘤的电镜观察可以明显地见到细胞内充满了细菌。乙炔还原法测定的固氨酶活性（C2H4）为76.80nmol／（plant·h）。
Nodular structures have been induced on oilseed rape root by treating the 3cm length roots of 2-d seedlings with a pectobacterium, followed by inoculation with a mutunt strain of Rhizobium astraglus, the nodulation rate is 96. 80%. The strains were isolated from nodules of oilseed rape and successfully reinoculated to original host.Transverse section of para-nodules showed that cells of nodule were full of bacteria.The activity of the nodules was determined by acetylene reduction method (C2H4,76. 80 nmol·plant-1·h-1).
出处
《植物营养与肥料学报》
CAS
CSCD
1997年第3期275-279,共5页
Journal of Plant Nutrition and Fertilizers
基金
总理基金
英国皇家学会资助
