摘要
目的探讨去卵巢大鼠血清中活性氧及抗氧化体系的变化,为临床早期诊断及治疗骨质疏松提供依据。方法选用4月龄清洁级SD大鼠20只,随机分为正常对照组和模型组。模型组行双侧卵巢切除术,对照组行假手术。三个月后测量两组大鼠体重、子宫湿重、左侧股骨及腰椎骨密度、左侧股骨生物力学及血清生化指标Ca2+、肌酐(Cr)、碱性磷酸酶(ALP)、抑制OH能力、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的水平。结果模型组与对照组相比,体重明显增加,股骨及腰椎骨密度均降低,股骨生物力学特性最大载荷降低,子宫湿重降低,血清Ca2+、ALP、MDA、Cr水平升高,抑制OH能力、SOD、GSH-Px水平降低,差异有统计学意义(P<0.05)。结论血清中活性氧及抗氧化成分羟自由基、SOD、MDA及GSH-Px等可能参与了绝经后骨质疏松的病理进程。
Objective To explore the changes of reactive oxygen species （ROS） and antioxidants in the serums of ovariectomized osteoporosis rats to provide the evidences for early diagnosis and therapy. Methods Twenty female SD rats aged 4 months were divided into control （sham operation） and model （ovariectomy） groups randomly. The body weight, uterus wet weight, BMD of left femurs and lumbar, biomechanical characteristics of left femurs, the levels of serum Ca^2＋ , Cr, alkaline phosphatase （ ALP）, SOD, MDA and GSH-Px and the serums＇ resisting abilities to OH^- were measured 8 weeks later. Results 3 months after ovariectomy, the body weight increased markedly, BMD of left femurs and lumbar, the biomechanical maximal load and uterus weight decreased, the serum level of Ca^2 ＋ , ALP, MDA, GSH- Px increased, while SOD level and serum resisting abilities to OH- decreased in model group compared with those in control group （ P 〈 0. 05）. But Cr level didn＇t change. Conclusions ROS and antioxidants such as OH^-, SOD, MDA and GSH-Px might be involved in the pathological progress of postmenopausal osteoporosis.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2007年第22期2164-2166,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金(30300397)
广东省卫生厅基金(WSTJJ20041201170453519721104881)
关键词
骨质疏松
活性氧
骨密度
Reactive oxygen species （ROS）
Osteoporosis
Bone mineral density （BMD）
