摘要
目的:建立浊度法测定注射用硫酸奈替米星的含量并进行方法学验证。方法:以金黄色葡萄球菌制作菌悬液,接种于抗生素检定Ⅲ号培养基中,取9 mL 培养液和1 mL 不同浓度的硫酸奈替米星置比色管中,37℃培养4 h 左右,530 nm 在线测定。结果:培养物浊度与奈替米星浓度的 log 值在0.6～1.6 u·mL^(-1)之间呈线性关系(r>0.99);测得奈替米星的平均回收率为100.7%(RSD=3.4%)。结论:该方法准确、可靠、灵敏度高,耗时短。
Objective ：To establish turbidimetric method with determination Netilmicin Sulfate for injection. Method： Inoculate medium IU with a suspension of Staphylococcus aureus, Distribute 9 mL of the inoculated medium and 1 mL of the Netilmiein Sulfate series of doses＇ solution into test - tubes, determine on - linely on 530 nm after an incubation period of about 4 h at 37 ℃. Result： Linearity of the dose - response relationship about the turbidity and the antibiotic doses＇ log was between 0. 6 - 1.6 u · mL^-1 （r 〉0. 99） ;The average recovery was 100. 7% （ RSD = 3.4% ）. Conclusion ：The method is accurate, reliable, sensitive and quick.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第11期1816-1819,共4页
Chinese Journal of Pharmaceutical Analysis
