摘要
目的:采用 HPLC 法建立岩黄连药材指纹图谱。方法:应用 Shim—pack CLC—ODS 色谱柱(6.0 mm×150 mm,5μm),乙腈-缓冲溶液[0.05 mol·L^(-1)庚烷磺酸钠-0.05 mol·L^(-1)磷酸二氢钾(1:1),含0.2%乙二胺,用磷酸调 pH 3.0]梯度洗脱:0～130 min,流动相比例20:80→27:73;130～140 min,比例27:73→20:80;140～160 min,比例20:80。流速为1 mL·min^(-1),检测波长285 nm,柱温:室温。以中药色谱指纹图谱相似度评价系统的操作规范(版本2004 A)软件计算。结果:10批药材的相似度在0.765～0.989,确定了8个共有峰,建立了岩黄连药材 HPLC 指纹图谱。结论:本法可作为岩黄连药材的质量控制方法。
Objective：To establish HPLC fingerprint of Herba Corydalis Saxicolae. Method：Chromatographic condition included a Shim - pack CLC - ODS（6. 0 mm × 150 mm,5 μm）column and the gradient was adopted elution with acetonitrile- buffer solution [ 0. 05 mol·L^-1 sodium heptanesulfonate -0. 05 mol· L^-1 potassium dihydrogen phos- phate（ 1：1 ）, contained 0. 2% diethylamine, adjust to pH 3.0 with phosphoric acid ], and gradient models were mobile phase from 20：80 to 27：73 in 0 - 130 min,then from 27：73 to 20：80 in 130 - 140 min,20：80 maintain for 20 min. The detection wavelength was at 285 nm and the flow rate was 1.0 mL· min^- 1, the column temperature was at room temperature. Use similarty evaluation system for chromatographic fingerprint of TCM to evaluate. Results： Similarity of 10 batches sample were 0. 765 - 0. 989, the fingerprint of Herba Corydalis Saxicolae was established and 8 common peaks were indicated. Conclusion：This method can be used for qualitaty control of Herba Corydalis Saxicolae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第11期1749-1751,共3页
Chinese Journal of Pharmaceutical Analysis
基金
广西科学基金(0448046)
广西"新世纪十百千人才工程"专项资金资助项目(2003226)
