摘要
目的:探讨神经肽Y(NPY)在妊娠肝内胆汁淤积症(ICP)不同临床分度患者胎盘中的表达及其临床意义。方法:将60例ICP患者分为轻度ICP、中度ICP、重度ICP3组,每组20例,采用免疫组化方法检测患者胎盘中NPY的表达并进行定位分析,通过计算机图像系统分析NPY表达的程度及强度,同时进行半定量分析。结果:(1)NPY表达于胞浆或胞膜上。(2)NPY在轻度、中度、重度ICP组的平均光密度值(OD值)分别为0.9230±0.1524、1.0738±0.1493、1.6003±0.1182。重度ICP组NPY胎盘中的表达高于中度ICP组与轻度ICP组(q分别为5.73和9.61,P<0.01),轻度组与中度组间差别无统计学意义(q=2.95,P>0.05)。结论:NPY的表达异常及水平高低可能与ICP患者的病情程度及预后有关。
Objective： To investigate the expression and clinical significance of NPY of the placenta of ICP in various clinic stages. Methods： Sixty ICP patients were randomly divided into three groups including mild group（20）,moderate group（20） and severe group（20）. Expression and quantification of NPY women were detected by immunohistochemistry staining and image analysis. The expression levels of NPY were compared. Results： （1）NPY protein was expressed in cell plasma. （2）Average value of optical density （OD） of NPY by image analysis in mild, moderate and severe ICP was 0.923 0 ± 0.152 4,1.073 8 ± 0.149 3 and 1.600 3 ± 0.118 2 respectively. The expression level of NPY was higher in severe ICP group than those in the other two groups. No significant difference was found between the mild and moderate ICP. Conclusion： The abnormal expression of NPY may play a crucial role in the pathogenesis of ICP placenta.
出处
《天津医药》
CAS
北大核心
2007年第11期832-833,F0003,共3页
Tianjin Medical Journal
关键词
妊娠并发症
胆汁淤积
肝内
胎盘
神经肽Y
免疫组织化学
pregnancy complications cholestasis,intrahepatic placenta neuropeptide Y immunohistochemistry
