摘要
目的:观察碱性成纤维生长因子(bFGF)处理的缺血再灌注不同时程的猫脑组织中微管相关蛋白(MAP-2)和神经丝蛋白(NTP)的表达,探讨bFGF治疗缺血性脑损伤的可能作用机制。方法:健康家猫30只,随机分为生理盐水对照组和bFGF治疗组。采用左侧眼眶入路制作大脑中动脉缺血再灌注模型。于术前和再灌注24h、48h和7d,采用Philip的猫脑缺血神经功能评分标准进行神经功能缺损评分;应用免疫组织化学SP法检测缺血再灌注不同时程的脑组织MAP-2及NTP蛋白表达,进行免疫阳性细胞计数。结果:缺血再灌注48h后,治疗组动物神经功能受损程度较对照组明显减轻,MAP-2及NTP蛋白阳性细胞数目较对照组也显著增加。结论:bFGF通过诱导MAP-2及NTP蛋白的表达,减轻了缺血再灌注脑组织的神经元损伤和促进了神经纤维生长,从而改善受损的神经功能。
Aim： To observe the expressions of microtubule associated protein 2 （MAP-2） and neural thread protein （NTP） in brains of acute cerebral infarction cats treated by using basic fibroblast growth factor （bFGF）, and to explore a possible mechanism of bFGF action for treating ischemic cerebral injury. Methods： 30 healthy adult cats were randomly and equally divided into the control group and bFGF-treated group. All animals were operated to establish the feline models of cerebral focal ischemia-reperfusion by the approach of left transorbital. The score of neurological defects was evaluated by Philip＇s criteria score, and the expressions of MAP-2 and NTP were observed by immunohistochemisty before operation and after ischemia for 2 h and reperfusion for 24 h, 48 h and 7 d. The number of the positive cells was calculated under the microscope. Results ： After ischemia/reperfusion for 48 h, the score of neurological defects was obviously decreased, but both of MAP-2 and NTP positive cellular numbers were distinctly increased in the cat＇s brains of bFGF- treated group compared with the animals of control group. Conclusion： bFGF had a remarkable therapeutic effect to acute cerebral infarction by inducing MAP-2 and NTP expressions, which can relieve the neuronal injury caused by ischemia/reperfusion, accelerate the growth of nerve fibers and improve the nervous function.
出处
《中国临床神经科学》
2007年第6期633-637,共5页
Chinese Journal of Clinical Neurosciences
关键词
脑梗死
碱性成纤维细胞生长因子
微管相关蛋白
神经丝蛋白
cerebral infarction
basic fibroblast growth factor
microtubule associated protein 2
neural thread protein
