摘要
目的:观察小分子干扰RNA(siRNA)对人食管癌EC9706细胞中血管内皮生长因子C(VEGF-C)表达的抑制作用。方法:根据VEGF-C cDNA已知序列,设计并体外转录合成3条siRNA,将它们分别导入载体质粒中,用脂质体介导分别转染EC9706细胞,以无关序列转染和正常EC9706细胞为对照。采用免疫细胞化学SP法、半定量RT-PCR和原位杂交技术分别检测上述各组细胞中VEGF-C蛋白和mRNA的表达。结果:正常细胞对照组和无关序列转染组均有大量VEGF-C蛋白阳性颗粒和mRNA阳性条带,而在siRNA转染的3组中,仅有少量VEGF-C阳性表达颗粒和较弱的mRNA阳性条带,与正常细胞对照组和无关序列转染组相比,差异均有统计学意义(P<0.01)。结论:VEGF-C siRNA能有效抑制食管癌EC9706细胞中VEGF-C的表达,可望成为一种抗淋巴管生成的新方法。
Aim ： To observe the inhibition of small interfering RNA （siRNA） on the expression of vascular endothelial growth factor C （VEGF-C） in human esophageal cancer EC9706 ceils. Methods： Three self-designed and constructed VEGF-C-targeted siRNA eukaryotic expression vectors were introduced into esophageal carcinoma EC9706 cells via lipofectin mediation. The expressions of VEGF-C protein and mRNA in EC9706 cells were observed by SP immunocytochemistry,semi-quantitative RT-PCR and in situ hybridization, respectively. The normal EC9706 cells that were transfected with unrelated sequence were used as control. Results： There were lots of VEGF positive granules and positive bands in normal EC9706 cells and in the transfection group transfected with unrelated sequence. The cells in transfection group transfected by VEGF-C-targeted siRNA expressed few positive granules and weak positive bands. There was significant difference between them （P 〈 0.01 ）. Conclusion： The specific siRNA of VEGF-C can significantly inhibit the expression of VEGF-C in EC9706 cells. The siRNA against VEGF-C may serve as a potential method for cancer treatment of human esophageal cancer.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2007年第6期1022-1024,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
"十五""211工程"重点学科建设基金资助项目教重办200202
河南省高校杰出科研人才创新工程基金资助项目2006KYCX016
关键词
EC9706细胞
VEGF—C
SIRNA
EC9706 cell
vascular endothelial growth factor C
small interfering RNA
