摘要
为了研究HAP纳米粒子对肝癌细胞的增殖能力及生物学行为的影响，采用均匀沉淀法制备了均匀分散的纳米尺度的HAP纳米粒子。以0．56mmol/的HAP纳米粒子与Bel-7402肝癌细胞作用4d后，细胞行Feulgen染色和AgNOR染色，显微镜观察标本、图像分析系统定量分析肝癌细胞的DNA和AgNOR。结果显示HAP纳米粒子能降低Bel-7402细胞的DNA含量，减少细胞的AgNOR数量，与对照纽比较均有显著性差异（P〈0．01）。HAP纳米粒子能减弱肝癌细胞的增殖活性，可能是通过抑制DNA的合成，以及减少AgNOR数量而抑制rRNA的合成，发挥抗癌作用。
To investigate the effect of hydroxyapatite nanoparticles on proliferative potential and biological behaviour of the human hepatoceUular carcinoma, HAP nanoparticles were synthesized with the homogeneous precipitation method, and their shape and dispersity observed by TEM was homogeneous, rod-shaped. After the treatment of Bel-7402 hepatocellular carcinoma cells with HAP nanoparticles at a concentration of 0.56 mmol/L for 4 days, Feulgen and AgNOR stain were conducted and the specimens were observed by microscope. DNA and AgNOR were quantified with image analysis techniques. It was found that compared to the control, there were a significant decrease of the DNA content （P〈0.01） and an obvious decrease in the number of AgNOR granules （P〈0.01）. The results showed that HAP nanoparticles could decrease the DNA reproductive activity and reduce the rRNA synthesis in Bel-7402 cells, and thus is likely able to inhibit the proliferation of hepatocellular carcinoma.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2007年第A05期1746-1747,共2页
Journal of Functional Materials
基金
复合材料新技术国家重点实验室资助项目（WUT2004202）
