摘要
利用RAPD分析和BOXAIR-PCR技术对分离自我国天津、河北、山东、黑龙江三江平原等地盐碱土的43株硅酸盐细菌及3株参比菌株进行了遗传多样性研究。结果表明,供试盐碱土硅酸盐细菌间存在较大的遗传多样性,对6个单引物和2个双引物进行的RAPD扩增,供试引物分别产生了1～13条谱带,聚类分析在82%的相似水平上将供试菌株聚为13个群;在75%的相似水平处,BOXAIRPCR将供试菌株聚为12个遗传群。两种分子标记技术均较好地揭示了盐碱土硅酸盐细菌的遗传多样性特征。
Forty three silicate bacteria isolated from saline-alkali soils collected from Tianjing, Hebei, Shandong and Heilongjiang Provinces in northern China, together with three reference strains, were analyzed by RAPD （including single primer RAPD and Two-primer RAPDs ） and BOXAIR-PCR. Considering the stability of RAPD, 6 single RAPD primers and 2 TP-PCR primers were choosed for analysis of genetic diversity. The results demonstrated that the genetic diversity was existed among the silicate bacteria, and there were about 1 to 13 fingerprint bands amplified by RAPD, and the results of dendrogram generated by RAPD patterns showed that, at similarity of 82%, 46 silicate strains were divided into 13 genetic groups： group Ⅰ, group Ⅱ and group Ⅲ were almost constituted by the silicate bacterium strains that came from Heilongjiang, which showed the geographical distribution of the srains in this region, but most of the genetic groups were not obviously related to the geographical origin. Based on BOXAIR PCR, 12 genetic groups were formed at similarity of 75%. 13 amplified fragments bands and 10 genotypes were detected with BOXAIR primer, demonstrating that there were great genetic differentiations among the tested silicate bacteria and that there was no correlation between genetic groups and the geographical origin. Both RAPDs and BOXAIR PCR revealed the genetic differentiations among the the tested silicate bacteria properly.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2007年第6期2043-2047,共5页
Journal of Agro-Environment Science
基金
四川农业大学青年科技创新基金(14304)
华中农业大学农业微生物学国家重点实验室基金资助
