摘要
小鹅瘟是严重危害养鹅业的主要疾病之一，自５０年代发现已来，世界各国都在探讨诊断和防治该病的安全有效而又经济方便的方法。我们利用淋巴细胞杂交瘤技术研制出１１株能稳定分泌小鹅瘟病毒单抗的杂交瘤细胞系，并在对其特异性鉴定、琼扩沉淀效价、鹅胚内及实验雏鹅的中和效果及实验性防治效果测定的基础上，选择其中２株高沉淀效价和中和效价的单抗（ＧＰＡ１和ＧＰＣ５）进行了较大规模的（计１３６３５只雏鹅）现地预防和治疗小鹅瘟试验，其存活率分别达到９８．２％和９０．５％，获得了良好的防治效果，具有很高的临床应用价值。
Gosling Plague was one of the severe diseases which endangered Goose farm Safe,effective and economical methods of diagnosis,prevention and treatment for the disease have been explored in the World after Gosling Plague was discovered in 1950s Eleven hybridoma cell lines,secreting monoclonal antibodies(MAbs) against Goose Parvovirus(GPV),were developed by lymphocyte hybridoma technique The specificity and agar precipitation titer of these MAbs were identified Atter neutralizing ability was detected in Goose embryos and chickens ability prevention and treatment experiment with these MAbs for Gosling Plague was carried out Basedon these results,two monoclonal antibodies(GPA1 and GPC5),secreting high titers of agar diffusion and neutralizing antibodics,were used in erperiment for preventin andtreatmeut for Gosling Plague by large scale(total 13,636 chickens)in field The protective rates were 98 2 and 90 5 percent,respectively good effect of prevention and treatment was obtainek These MAbs were proved to be effective in clinical application
基金
江苏省科委资助
