摘要
以待检测的寡核苷酸本身作为一个引物，加上两个载体特异引物，组成两对PCR引物。含待检测寡核苷酸片段的重组DNA用这两对引物可分别扩增出两个大小不同的片段，而载体DNA只有一对引物（即载体特异引物）可扩增出一个较小的片段。
The recombinant DNA clones containing specific oligonucleotide can be identified conveniently and rapidly by a non-radioactive polymerase chain reaction (PCR) based method. In this methed, the oligonucleotide which is to be identified serves as a primer of the three primerswhich constitute two primer pairs. The other two primers are specific for the sequence of the vector. Two PCR products which have different sizes can be generated from positive recombinant DNA with two primer pairs respetively, whereas only a primer pair specific for the vector produces successful amplification from vector DNA.
出处
《生物技术》
CAS
CSCD
1997年第4期11-12,共2页
Biotechnology
基金
广东省科委青年科学基金
