摘要
应用RACE-PCR技术,克隆了全长3159 bp鹅催乳素受体基因(gPRLR)cDNA。序列分析表明,cDNA含443 bp5-′UTR、2496 bp编码区(含终止密码子TAA)和220 bp 3-′UTR。比对鸡催乳素受体基因并将前330 bp看作第1外显子,则gPRLR基因可划分为14个外显子。推导的蛋白序列含831个氨基酸,与鸡、鸽及火鸡PRLR的同源性分别为87.7%、85.2%和84.8%,其N末端含24个氨基酸的信号肽,成熟蛋白含807个氨基酸。gPRLR mRNA在成年鹅睾丸、输精管、卵巢、输卵管、肾、大肠、小肠、脾组织中均有表达,其中以肾、睾丸、大肠及小肠中表达最为丰富。
The goose prolactin receptor （gPRLR） cDNA with a length of 3 159 bp were cloned by RACE-PCR, the result indicated that the cDNA contained 443 bp 5＇-UTR, 2 496 bp with sequence containing TAA stop codon and 220 bp 3＇-UTR. Compared with cPRLR cDNA, the cDNA was presumably composed of 14 exons （if the first 330 bp is the first exon）. The putative protein contained 831 amino acids and exhibited identities of 87. 7%, 85.2% and 84. 8% with chicken, pigeon and turkey PRLRs, respectively. N-terminus of the protein comprised a signal peptide of 24 amino acids and the mature protein is 807 amino acid long. RT-PCR analysis showed that the gPRLR mRNA was widely expressed in testis, seminiferous duct, kidney, ovary, oviduct, large intestine, small intestine and spleen, and the highest abundance was presented in kidney, oviduct, large intestine and small intestine.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2007年第4期97-101,共5页
Journal of Nanjing Agricultural University
基金
江苏省自然科学基金项目(BK2004173)
