丹参酮ⅡA对人卵巢癌细胞株CAOV_3增殖与凋亡的影响被引量：17

Effects of Tanshinone ⅡA on the Proliferation and Apoptosis of Human Ovarian Cancer Cell Line CAOV_3

导出

摘要目的观察丹参酮ⅡA对人卵巢癌细胞株(CAOV3)增殖与凋亡的影响。方法培养人卵巢癌细胞株(CAOV3),四甲基偶氮唑盐(MTT)比色法测定丹参酮ⅡA对细胞增殖的抑制作用;免疫沉淀法纯化蛋白,细胞外信号调节激酶(ERK)试剂盒测定ERK活性;流式细胞术测定丹参酮ⅡA对细胞凋亡的影响;免疫印记法(W estern-b lot)测定Bax及bc l-2表达。结果MTT实验显示丹参酮ⅡA对CAOV3细胞增殖有明显抑制作用,免疫沉淀法显示丹参酮ⅡA可抑制ERK活性,流式细胞术显示丹参酮ⅡA可诱导CAOV3细胞凋亡,免疫印记法显示丹参酮ⅡA上调Bax,同时降低bc l-2表达,使Bax/bc l-2比值增加。结论丹参酮ⅡA可通过抑制ERK通路而抑制卵巢癌细胞增殖,并诱导细胞凋亡,其途径与上调Bax表达,降低bc l-2表达有关。 Objective To study the effects of Tanshinone Ⅱ A （Tan Ⅱ A） on the proliferation and apoptosis of human ovarian cancer cell line CAOV3. Methods The human ovarian cancer cell line CAOV3 was cultured in vitro. The effect of Tan ⅡA on the proliferation of the CAOV3 cells was assayed with the MTT colorimetric method. ERK （ extracellular signal - regulated kinase） activity was measured according to the directions of the ERK kit after the protein immuno - precipitation. The effect of Tan Ⅱ A on the apoptosis of the cells was determined with flow cytometry. The expression of Bax and bcl-2 was analyzed with Western blot. Results The MTT test showed that Tan Ⅱ A exerted a striking inhibitory effect on the proliferation of CAOV3 cells in a dosage dependent manner. Tan Ⅱ A was also shown to depress the ERK activity after the immuno-precipitation of the protein. Flow cytometry revealed that Tan Ⅱ A induced apoptosis of the CAOV3 cells. Western blot showed that Bax expression was up-regulated while bcl-2 expression was down-regulated in the CAOV3 cells treated with Tan Ⅱ A, thus increasing the ratio of Bax/bcl-2. Conclusion Tan Ⅱ A was shown to depress the proliferation and induce the apoptosis of the CAOV3 cells by inhibiting the ERK activity. The signal transduction may be related to the up-regulation of the Bax expression and downregulation of the Bcl-2 expression.

作者刘伟陈昊

机构地区湖北省直属机关医院药剂科华中科技大学同济医学院医药卫生管理学院

出处《医药导报》 CAS 2007年第12期1398-1400,共3页 Herald of Medicine

关键词丹参酮ⅡA 卵巢癌增殖凋亡 Sodium Tanshinone Ⅱ A sulfonate Ovarian cancer Proliferation Apoptosis

分类号 R282.71 [医药卫生—中药学] R285.5 [医药卫生—中药学]

引文网络
相关文献

参考文献7

1ZHAO B L, JIANG W, ZHAO Y, et al. Scavenging effects of salvia mihiorrhiza on free radicals and its protection for myocardial mitochondrial membranes from ischemiareperfusion injury [ J ]. Biochem Mol Biol Int, 1996, 38(6) :1171 -1174.
2邓惠,罗焕敏,高勤,李晓光,肖飞,章佩芬,翁文.丹参酮ⅡA对U251胶质瘤细胞的增殖抑制和原癌基因表达的影响[J].中国病理生理杂志,2004,20(6):981-984. 被引量：12
3丁罡,宋明志,于尔辛.丹参、赤芍对大鼠Walker 256癌肝转移影响机制的研究[J].中国癌症杂志,2001,11(4):364-366. 被引量：138
4WEBB C P, VAN AELST L, WIGLER M H, et al. Signaling pathways in Ras-mediated tumorigenicity and metastasis [ J ], Proc Natl Acad Sci, 1998, 95 ( 15 ) : 8773 - 8778.
5WEINSTEIN-OPPENHEIMER C R, BLALOCK W L, STEEMAN L S, et al, The raf signal transduction cascade as a target for chemotherapeutic intervention in growth factorresponsive tumour [ J ]. Pharmacol Ther, 2000, 88 ( 3 ) : 229 - 279,
6ADAMS J M , CORY S. The bcl-2 protein family: arbiters of cell survival[J]. Science, 1998, 281 (5381): 1322- 1326.
7REED J C, Bcl-2 family proteins[J], Oncogene , 1998 , 17 (25) : 3225 - 3236.

二级参考文献12

1李承珠,杨诗春,赵风娣,黄春妹.活血化瘀中药丹参对大鼠Walker256癌细胞血行播散的影响[J].肿瘤,1983(2):66-66. 被引量：1
2Chang WL,Chen CF.Cytotoxic activity of Tanshinone against human carcinoma cell lines[J].Am J Chin Med,1991,19(3):207-212.
3Israels ED,Israels LG.The cell cycle[J].Stem Cells,2001,19(1):88-93.
4Wagner AJ,Kokontis JM,Hay N.Myc-mediated apoptosis requires wild-type p53 in a manner independent of cell cycle arrest and the ability of p53 to induce p21[J].Genes Dev,1994,8(2):2817-2830.
5Mooy CM,Luyten GP,de Jong PT,et al.Immunohistochemical and prognostic analysis of apoptosis and proliferation in uvesl melanoma[J].Am J Pathol,1995,147(5):1097-1104.
6Mink S,Mutschler B,Weiskirchen R,et al.A novel function for Myc:inhibition of C/EBP-dependent gene activation[J].Proc Nall Acad Sci USA,1996,93(3):6635-6640.
7Packham G,Cleveland JL.C-Myc and apoptosis[J].Biochim Biophys Acta,1995,1242(1):11-28.
8Bacon CL.Antiproliferative action of valproate is associated with aberrant expression and nuclear translocation of cyclin D3 during the C6 glioma Ghase[J].J Ncurochem,2002,83(1):12-19.
9Eilers M,Didler P,Kelth Y,et al.imaeras ofMyc oncoprotein and steroid receptors cause hormone-dependent transformation of cells[J].Nature,1989,340(6):66-68.
10Somay C,Gnut TW,Mannhalter C,et al.Relationship of myc protein expression to the phenotype and to the growth potential of HOC-7 ovarian cancer cells [J].Br J Cancer,1992,66(1):93-98.