摘要
高质量的微生物基因组DNA是基因工程的前提。目前国内外关于微生物基因组DNA提取的方法很多,根据研究对象和目的不同而方法各异。该文就现有方法中应用最为广泛的三种提取微生物基因组DNA的方法进行了比较,并对它们进行一些改进,获得了针对不同细胞壁成分的微生物相应的简便、快速且高质量基因组DNA提取方法,并对提取的DNA进行PCR特异性扩增检测,获得较清晰的谱带[1],为基因克隆表达研究奠定了基础。
High quality genomic DNA from microorganism is the precondition of genetic manipulation. Now many methods for the extraction of genomic DNA are developed according to different research object and intention. Three kinds of methods widely applied are compared and reformed in isolation of genomic DNA from microorganism. And effective methods for extracting high pure genomic DNA are established considering different cell wall components. Genomic DNA gained by three different methods above is specifically amplified and clear band is observed by agarose gel electrophoresis, which is convenient to genetic manipulation later.
出处
《嘉兴学院学报》
2007年第3期48-50,共3页
Journal of Jiaxing University
关键词
微生物
DNA提取
PCR
microorganism
DNA extraction
PCR （ Polymerase Chain Reaction ）
