摘要
目的:研究Hepsin基因对前列腺癌PC3细胞生物学特性的影响。方法:用脂质体介导的基因转染技术,将pHepsin-IRES2质粒转染前列腺癌PC3细胞,用G418(600mg/L)筛选出阳性克隆。实时PCR检测转染前后Hepsin表达;MTT法和BrdU法检测细胞生长曲线;Boyden小室和划痕法观察转染前后细胞侵袭力的变化。结果:建立稳定表达Hepsin基因的PC3细胞系;转染Hepsin基因的PC3细胞的生长速度和转染空载体的对照组没有显著差异;转染Hepsin基因后前列腺癌PC3细胞的侵袭力显著增加(P<0.05)。结论:Hepsin基因对前列腺癌PC3细胞的增殖无显著作用,可明显促进PC3细胞的侵袭力。
Objective：To investigate the effects of Hepsin gene on the biological behavior of prostate cancer PC3 cells. Methods：Plasmid pHepsin-IRES2 was transfeeted into prostate cancer PC3 cells by Fugen6, and cells that can express Hepsin stably was screened out by G418（600 mg/L）. Hepsin mRNA levels were measured by Real-time PCR; Growth curve of the Hepsin gene transfected PC3 cells was drawn with the results of MTT and BrdU assay The Boyden chamber and wound healing were used to detect the difference of invasion and metastases between transfected and non-transfected cells. Results： Hepsin mRNA level in pHepsin-IRES2 transfected PC3 cells was significantly higher than that in control PC3 cells; The growth curve of the Hepsin gene transfected was cells by MTT and BrdU revealed that the Hepsin gene transfected PC3 cells had no significant effect in proliferation. As compared with control cells, the invasion ability of pHepsin-IRES2 transfected PC3 cells was increased obviously （P〈0.05）. Conclusions： Hepsin, a type H transmembrane serine protease, has no impact on cell prolifera- tion, but promotes prostate cancer PC3 cells invasion and metastasis.
出处
《临床泌尿外科杂志》
2007年第11期861-863,共3页
Journal of Clinical Urology
关键词
前列腺癌
增殖
肿瘤侵袭
生物学
Prostate carcinoma
Proliferation
Metastasis Biology
