摘要
采用5 mW·mm-2的He-Ne激光,10.08 kJ·m-2·d-1的太阳紫外线B辐射(UV-B)及二者的组合对晋麦8号小麦幼苗进行处理,5 d后测定小麦幼苗叶片中超氧阴离子的产生速率、丙二醛(MDA)含量、小麦幼苗叶片浸出液的紫外吸收值和电导率、可溶性蛋白含量及叶绿素含量的变化,分析He-Ne激光对增强UV-B辐射引起小麦损伤的修复效应.结果显示,与UV-B辐射处理比较,He-Ne激光辐照可使小麦幼苗叶片中超氧阴离子的产生速率减小,丙二醛含量减少,叶片浸出液的紫外吸收值和电导率降低,可溶性蛋白质量分数增加到76.66%和叶绿素质量比升高至1.79 mg/g.表明超氧阴离子的产生速率、丙二醛、小麦幼苗叶片浸出液的紫外吸收值和电导率、可溶性蛋白、叶绿素变化同小麦幼苗损伤修复能力相关,从而证明一定剂量的He-Ne激光可以部分修复增强UV-B造成小麦幼苗的辐射损伤.
The seedlings of wheat （Jinmai 8） were exposed to He-Ne laser with 5 mW·mm^-2 power density, enhanced ultraviolet-B radiation （UV-B） （10.08 kJ·m^-2·d^-1 ） and the combination of He-Ne laser and enhanced UV-B radiation respectively for 5 days. Changes about production rate of superoxide anion, malondialdehyde （MDA） content, ultraviolet absorption value and conductance of exosmic fluid of wheat seedlings, content of soluble protein and chlorophyll were measured to analyze the repair role of He-Ne laser irradiation. The results showed that He-Ne laser irradiation on the wheat seedlings led to the decrease of the production rate of superoxide anion, MDA content and ultraviolet absorption value and conductance of exosmic fluid of wheat seedlings. The content of soluble protein and chlorophyll increased to 76.66% and 1.79 mg/g respectively. It suggested that those changes about production rate of superoxide anion, MDA, ultraviolet absorbing value and conductance, soluble protein and chlorophyll were related to the repair capacity of the wheat seedings. Therefore, the damage of wheat seedlings induced by enhanced UV-B radiation can be repaired partly by He-Ne laser.
出处
《中国激光》
EI
CAS
CSCD
北大核心
2007年第11期1594-1599,共6页
Chinese Journal of Lasers
基金
国家自然科学基金(30671061)
山西省自然科学基金(20041101)资助项目
