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COX-2表达对脑胶质瘤的放射敏感性和细胞周期的影响 被引量：4

The impact of COX-2 expression on the radiosensitivity and cell cycle distribution in human glioma cell
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摘要 背景与目的:COX-2在胶质瘤的发生发展过程中发挥重要作用,近年来发现COX-2抑制剂对一些胶质瘤细胞有增强放射敏感性的作用。本研究的目的在于观察人恶性脑胶质瘤细胞的COX-2蛋白表达和COX-1/COX-2双重抑制剂对乙酰氨基酚(acetaminophen,ACE)对细胞COX-2mRNA表达、克隆形成率和细胞周期的影响,并探讨ACE提高胶质瘤细胞放射敏感性的机制。方法:选用人恶性脑胶质瘤细胞株SHG-44作为研究对象。免疫细胞化学和RT-PCR检测细胞COX-2表达,成克隆分析法测定对乙酰氨基酚对细胞的放射增敏作用,流式细胞仪检测细胞周期分布。结果:免疫细胞化学染色SHG-44细胞的胞膜和胞质均有COX-2蛋白表达。RT-PCR检测对乙酰氨基酚作用后细胞中COX-2mRNA表达降低。对乙酰氨基酚联合放疗作用于SHG-44细胞与单纯照射组相比,显示了放射增敏作用,增敏比为1.23(D0值水平)或1.43(Dq值水平)。细胞周期检测发现,不同时间各组细胞都出现了不同的周期分布,照射后12h单纯照射组和放射加药组G2/M期细胞明显多于对照组,24h后单纯照射组迅速下降到与对照组相等的水平,而放射加药组仍维持较高比例(P<0.01),同时对放射抗拒的S期细胞比例最低。结论:人恶性脑胶质瘤细胞株SHG-44的胞膜和胞质均有COX-2蛋白表达,对乙酰氨基酚能增加该细胞株的放射敏感性,其机制可能与抑制COX-2表达、改变细胞周期分布有关。 Background and purpose：COX-2 plays an important role in the progress of glioma.Recently some studies show COX-2 inhibitors enhance the response of glioma to irradiation.The aim of this paper is to observe the expression of COX-2 in the human glioma cell and the effects of acetaminophen（ACE）,a non-selective cyclooxygenase（COX）-2 inhibitor,on the SHG-44 cells in terms of the expression of COX-2 mRNA、clone efficiency and cell cycle.The mechanism of the radiosensitivity enhancement by the affection of ACE was also investigated.Methods：The human glioma cell SHG-44 was used as cell model.COX-2 mRNA and protein expression of SHG-44 were detected by RT-PCR and immunocytochemisty staining.Clongenic assay was used for radiation survival estimate.The cell cycle distribution was analyzed by flow cytometry.Results：COX-2 protein expression in cytoplasm and cell membrane of SHG-44 cells were demonstrated by the immunocytochemisty staining experiment.Lower expression of COX-2 mRNA in the SHG-44 cells was observed when cells were incubated with 1mmol/L acetaminophen.Acetaminophen could enhance tumor cell to irradiation,SER was 1.23（D0）or 1.43（Dq）by clonogenic assay.The G2/M arrest in R and R＋A groups were significant as compared to group C after 12 hr treatment（P〈0.01）,but the percentage of G2/M phase cells in R group decreased rapidly after 24 hr treatment and was the same as C group（P〉0.05）,while the percentage of G2/M phase cells in R＋A group still maintained a high leval（P〈0.01）.Conclusions：There was expression of COX-2 in human glioma cell SHG-44.Acetaminophen significantly enhances radiosensitivity of SHG-44 glioma cells,probably via COX-2 inhibition,G2/M arrest or other mechanisms.
作者 徐晓婷 周菊英 俞志英 周乐源 秦颂兵 王利利 李莉
机构地区 苏州大学附属第一医院放疗科
出处 《中国癌症杂志》 CAS CSCD 2007年第11期838-842,共5页 China Oncology
关键词 放射增敏 细胞周期 对乙酰氨基酚 胶质瘤 radiosensitization cell cycle acetaminophen glioma
分类号 R730.55 [医药卫生—肿瘤] R73-35 [医药卫生—肿瘤]
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参考文献11

  • 1Taketo MM. Cyclooxygenase-2 inhibitors in tumorigenesis (part Ⅰ) [ J ]. J Natl Cancer Inst, 1998,90 (20) : 1529-1536.
  • 2Joki T, Hesse O, Black PM, et al. Expression of cyclooxygenase-2 (COX-2) in human glioma and in vitro inhibition by a specific COX-2 inhibitor, NS-398 [ J ]. J Cancer Res, 2000,60 ( 17 ) : 4926-4931.
  • 3Shonn T,Philip J, Tofilon JM, et al. Cyclooxygenase-2 expression in human gliomas[ J]. J Cancer Res,2001,61 ( 1 ) :43754381.
  • 4Howe LR, Dannenberg AJ. A role for cyclooxygenase-2 inhibitors in the prevention and treatment of cancer [ J ]. Semin Oncol, 2002,29(3 Suppl 11) :111-119.
  • 5Dannenberg AJ, Altorki NK, Boyle JO, et al. Cyclooxygenase-2 : a pharmacological target for the prevention of cancer [ J ]. Lancet Oncol,2001,2 :544-555.
  • 6Casper D, Lekhraj R, Yaparpalvi U,et al. Acetaminophen selectively reduces glioma cell growth and increases radiosensitivity in culture [ J ]. J Neuro Oncol,2000 ,46 :215-229.
  • 7Nakata E, Mason KA, Hunter N, et al. Potentiation of tumor response to radiation or chemoradiation by selective cyclooxygenase-2 enzye inhibitors[ J ]. Int J Radiat Oncol Biol Phys ,2004,58 (2) :369-375.
  • 8Raju U, Nakata E, Yang P, et al. In vitro enhancement of tumor cell radiosensitivity by a selective inhibitor of cyclooxygenase-2 enzyme:mechanistic considerations [ J ]. Int J Radiat Oncol Biol Phys ,2002,54 ( 3 ) :886-894.
  • 9Lu X,Xie W,Read D,et al. Nonsteroidal anti-inflammatory drugs cause apoptosis and induce cyclooxygenases in chicken embryo fibroblasts[ J ]. Proc Natl Acad Sci USA, 1995,92:7961-7965.
  • 10张有成,王杉,张辉,梁斌,叶颖江.转录信号传导子和激活子3信号传导通路调控选择性环氧化酶2抑制剂抗结肠癌的机制[J].中华胃肠外科杂志,2004,7(5):404-406. 被引量：5

二级参考文献12

  • 1DuBois RN, Radhika A, Reddy BS, et al. Increased cyclooxygenase-2 levels in carcinogen-induced rat colonic tumors.Gastroenterology, 1996, 110: 1259-1262.
  • 2Marx J. Cancer research. Anti-inflammatories inhibit cancer growth-but how? Science,2001, 291: 581-582.
  • 3Brennan MM, Lambkin HA, Sheehan CD, et al. Detection of high-risk subtypes of human papillomavirus in cervical swabs:routine use of the digene hybrid capture assay and polymerase chain reaction analysis. Br J Biomed Sci, 2001,58: 24-29.
  • 4Niikura T, Saegusa Y, Nishikawa T, et al. Titanium-alloy particles induced cyclooxygenase-2 in human macrophage-like cells in vitro. Kobe J Med Sci, 2002, 48:115-123.
  • 5Li HL, Zhang HW, Chen DD, et al. JIE-522: a selective COX-2 inhibitor, inhibits cell proliferation and induces apoptosis in RL95-2 cells. Acta Pharmacol Sin, 2002, 23: 631-637.
  • 6Grandis JR, Drenning SD, Zeng Q, et al. Constitutive activation of Stat3 signaling abrogates apoptosis in squamous cell carcinogenesis in vivo. Proc Natl Acad Sci USA, 2000, 97, 4227-4232.
  • 7Li M, Wu X, Xu XC. Induction of apoptosis in colon cancer cells by cyclooxygenase-2 inhibitor NS-398 through a cytochrome c-dependent pathway. Clin Cancer Res, 2001, 7: 1010-1016.
  • 8Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248-254.
  • 9Thun MJ, Henley SJ, Patrono C. Nonsteroidal anti-inflammatory drugs as anticancer agents: mechanistic, pharmacologic, and clinical issues. J Natl Cancer Inst, 2002, 94: 252-266.
  • 10Sandler RS, Halabi S, Baron JA, et al. A randomized trial of aspirin to prevent colorectal adenomas in patients with previous colorectal cancer. N Engl J Med, 2003, 348: 883-890.

共引文献4

同被引文献39

  • 1姜涛,王平.COX-2与子宫内膜癌的关系[J].实用癌症杂志,2005,20(5):477-479. 被引量：2
  • 2Levine B,Kroemer G.Autophagy in the pathogenesis ofdisease[J].Cell,2008,132(1):27-42.
  • 3Daido S,Yamamoto A,Fujiwara K,et al.Inhibition of theDNA-dependent protein kinase catalytic subunit radiosensi-tizes malignant glioma cells by inducing autophagy[J].Cancer Res,2005,65(10):4368-4375.
  • 4Thorburn A.Apoptosis and autophagy:regulatory connec-tions between two supposedly different processes[J].Ap-optosis,2008,13(1):1-9.
  • 5Harris RE.Cyclooxygenase-2(cox-2)blockade in the che-moprevention of cancers of the colon,breast,prostate,andlung[J].Inflammopharmacology,2009,17(2):55-67.
  • 6Dittmann KH,Mayer C,Ohneseit PA,et al.Celecoxib in-duced tumor cell radiosensitization by inhibiting radiationinduced nuclear EGFR transport and DNA-repair:a COX-2 independent mechanism[J].Int J Radiat Oncol BiolPhys,2008,70(1):203-212.
  • 7Chen JC,Chen Y,Su,et al.Celecoxib increased ex-pression of 14-3-3sigma and induced apoptosis of gliomacells[J].Anticancer Res,2007,27(4B):2547-2554.
  • 8Grosch S,Tegeder I,Niederberger E,et al.COX-2 inde-pendent induction of cell cycle arrest and apoptosis in co-lon cancer cells by the selective COX-2 inhibitor celecoxib[J].FASEB J,2001,15(14):2742-2744.
  • 9Lum JJ,Bauer DE,Kong M,et al.Growth factor regulationof autophagy and cell survival in the absence of apoptosis[J].Cell,2005,120(2):237-248.
  • 10Hetz C.Apoptosis,necrosis and autophagy:from mecha-nisms to biomedical applications[J].Curr Mol Med,2008,8(2):76-77.

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中国癌症杂志
2007年 第11期

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