摘要
目的研究p53、bcl-2蛋白在胃癌及癌前病变中的表达与胃癌生物学行为的关系。方法采用免疫组化SP法,检测p53、bcl-2蛋白在60例胃癌、46例异型增生和20例正常胃黏膜中的表达情况。结果p53在正常胃黏膜不表达,异型增生表达率为20%,随胃黏膜异型增生程度的加重,p53表达逐渐增高,在胃癌中的阳性表达率为53%。bcl-2在胃癌中的阳性表达率为47%,在异型增生中表达率为48%,在正常胃黏膜中表达呈弱阳性。bcl-2表达与胃癌的分化程度有关(P<0.05),与淋巴结转移、浸润深度无关(P>0.05);p53表达与上述临床病理因素均无关。结论p53、bcl-2蛋白的表达与胃黏膜增殖和恶化密切相关,可作为评估胃癌预后的参考指标。
Objective It is to explore the relationship of biological behavior of gastric carcinoma and expression of p53 and bcl - 2 proteins in gastric carcinoma and precancerous lesion. Methods Expressions of p53 and bcl - 2 proteins in 20 cases of normal gastric mucosa, 46 samples of gastric intraepithelial neplasia and 60 samples of gastric adenocarcinoma were determined by immuno-histochemical staining SP method. Results There was no xpression of p53 in normal gastric mucosa. The positive expression rate of p53 in gastric intraepithelial neplasia was 20 %, and in gastric adenocarcinoma was 53 %. The rate above increased gradually when the grade of intraepithelial neplasia got serious gradually. The positive expression rate of bcl- 2 in gastric carcinoma is 47 %, and in intraepithelial neplasia was 48 %. In normal gastric mucosa the rate was low positive. The expression of bcl - 2 was related with the differentiation degree of gastric carcinoma （ P 〈 0.05 ）, but wash＇ t related with the depth of infiltration and lymph node metastasis of gastric adenocarcinoma （ P 〉 0.05 ）. The expression of p53 had no relationship with the clinical pathological factors above. Conclusion The expression of p53 and bcl - 2 is associated with proliferation and malignant transformation of gastric mucosa, so they might be used as reference factors for the prognosis of gastric carcinoma.
出处
《现代中西医结合杂志》
CAS
2007年第34期5071-5072,共2页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
胃癌
P53蛋白
BCL-2蛋白
免疫组织化学
gastric carcinoma
p53 gene protein
bcl-2 gene protein
immuno-histochemistry
