摘要
目的 :为了解湖北肿瘤患者弓形虫感染概况并探索新技术直接免疫固相凝集试验( DIFA)等 3项试验联合筛选诊断弓形虫感染的意义。方法 :使用 DIFA检测 10 0例肿瘤患者的弓形虫 Ig G抗体 ,阳性者用 Ig G- IFAT复查 ,再用 Toxo- Ig G/ Ig A/ Ig M- ISAGA检测。结果 :DIFA检测阳性率为 12 % ( 12 / 10 0 ) ,Ig G- IFAT检测符合率为 10 0 % ;Ig A可疑 1例 ;Ig M抗体阳性 4例 ,可疑 1例 ,其中 2例提示为活动性感染。合并弓形虫感染的 12例分布于肺癌、非何杰金氏淋巴瘤和乳腺癌患者中 ,其感染率分别为受检人数的 2 2 .2 % ( 6/ 2 7)、19.3% ( 5/ 2 6)及 7.1% ( 1/ 14)。结论 :DI-FA的敏感性和特异性均较佳 ,3种方法的联合应用适宜作弓形虫感染的常规筛选诊断。提示免疫功能降低的肿瘤患者 ,特别是肺癌和非何杰金氏淋巴瘤患者容易感染弓形虫病或促使潜伏的弓形虫病活动。
AIM:To survey the prevalence of toxoplasmosis in tumor patients in Hubei and to ex- amine the significance of direct immunofixation agglutination(DIFA) ,Ig G- IFAT,and IS- AGA for dignosing Toxoplasma infection.METHODS:Sera from1 0 0 tumor cases were test- ed by DIFA for detecting anti- Toxoplasma Ig G antibodies.The positive sera were re- exam- ined by Ig G- IFAT and then detected by Toxo- Ig G/ Ig A/ Ig M- ISAGA.RESUL TS:Using DI- FA,the total anti- Toxoplasma antibody positive rate was 1 2 % (1 2 / 1 0 0 ) .Using Ig G- IFAT, the corresponding rate was1 0 0 % ,with one case suspected to be Ig A positive.Four cases were Ig M positive,two of them were with active infection and one was dubious.The rates of Toxoplasma infection in patients with lung cancer,non- Hodgkin lymphoma and breastcancer were2 2 .2 % (6 / 2 7) ,1 9.3% (5 / 2 6 ) and7.1 % (1 / 1 4) ,respectively.CONCL USION:DIFA is more sensitive and specific for diagnosing Toxoplasma infection and that cancer patients are more susceptible to Toxoplasma infection.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1997年第2期86-88,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
弓形虫感染
肿瘤
免疫
凝集试验
检测
Toxoplasma infection,tumor,direct imm unofixation agglutination,ISAGA
