摘要
miRNA是一类新近发现的存在于真核细胞中非编码小RNA,可以调控基因转录后的表达。人们通过获取、检测和验证等方法来研究miRNA及其靶基因。尽管人们已经在各种生物中预测并证实了数百种miRNA,但是miRNA及其靶基因的明确作用机制和功能尚不是完全明了。现就miRNA检测方法学的研究进展作一综述。
MicroRNA （ miRNA）, as a class of newly discovered non-protein-coding RNA, is present in eukaryote cells and plays a critical post-transcriptional repressor role in the regulation of gene expression. Many methods have been developed for the harvesting, detection and identification of miRNA and its target genes. Although hundreds of miRNA has been predicted and demonstrated in animals and plants, their definite mechanism, function and target genes are still unknown. Here is a reviews of the research advances methodology of detecting miRNA.
出处
《医学研究生学报》
CAS
2007年第11期1198-1201,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30571824)
