摘要
本文采用硫酸鱼精蛋白沉淀方法，从感染肾综合征出血热病毒（HFRSV）的鼠脑和细胞中提取病毒蛋白，结果表明：鼠脑先后用鱼精蛋白1mg／ml和2mg／ml两次沉淀HFRSV蛋白时，细胞先后用鱼精蛋白4mg／ml和2mg／ml两次沉淀HFRSV蛋白时，效果均较好。SDS－PAGE电泳显示：提取的病毒蛋白分子量为67kD、55kD、45kD。Western－Blotting及ELISA表明：提取的病毒蛋白为病毒的特异性蛋白。血凝试验表明：从鼠脑及细胞中提取的病毒蛋白血凝效价分别为1：1024、1：4096。液闪计数可观察到[3H]-氨基葡萄糖只结合入了67KD和55kD两种蛋白。
In this paper,we report a method to purify viral proteins by protamine. The viral proteins were purified by different concentration protamine from that mice brains and cells infected by Hantaan virus strain 76-118.SDS -PAGE showed three protein bands with molecular weights of 67kD.55kD and 45kD respectively.WesternBlotting and ELISA showed that the proteins pursied by protamine were HFRSV specific ones. HA indicated that HA titer of the proteins purified from mice brains and cells were 1:1024 and 1:4096. Beckman scintillation counter showed that 55kD and 67kD proteins were labelled by [3H]-glucosamine.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1997年第3期41-43,共3页
Chinese Journal of Zoonoses
关键词
肾综合征出血热
病毒
病毒蛋白
硫酸鱼精蛋白
Haemorrhagic fever with renal syndrome,Viral protein,Protamine sulfate
