摘要
目的:探讨木犀草素对脂多糖(LPS)诱导RAW264.7细胞COX-2及mPGES-1表达的影响。方法:酶免疫测定法(EIA)检测木犀草素对PGE2生成的影响,逆转录聚合酶链反应(RT-PCR)检测COX-2及mPGES-1 mRNA的表达。免疫印迹法(Western blotting)检测COX-2及mPGES-1蛋白的表达。结果:木犀草素抑制LPS诱导的RAW264.7细胞PGE2的生成,同时下调LPS诱导的RAW264.7细胞COX-2及mPGES-1 mRNA和蛋白的表达。结论:木犀草素可以抑制PGE合成途径中两个诱导酶COX-2和mPGES-1的表达。
Objective： To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells. Methods： After being pretreated with different concentrations of luteolin for 30 min, and then incubated with 1μg/ml LPS for 12h, the effect of luteolin on the product of PGE2 in RAW264.7 cells was measured by enzyme immunoassay （EIA）, The mRNA expression of COX-2 and mPGES-1 in RAW264.7 ceils were analysed by RT-PCK The COX-2 and mPGES-1 protein expression in RAW264.7 ceils were analysed by western blotting, Results： Luteolin inhibited the LPS-induced PGE2 synthesis in RAW264. 7 cells, The mRNA and protein expression of COX-2 and mPGES-1 in RAW264.7 cells were also decreased by luteolin. Conclusion： Luteolin can inhibit significantly the expression of COX-2 and mPGES-1 in PGE2 synthetic pathway.
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1263-1266,共4页
Journal of Chinese Medicinal Materials
基金
湛江市科技攻关项目(编号:2004090)
广东医学院青年基金项目(编号:2004109)
