摘要
目的:应用HPLC法建立福建省地道药材枇杷叶的化学指纹图谱。方法:采用HP1100高效液相色谱仪,色谱柱为Ec lipse XDB-C18(4.6 mm×150 mm,5μm);流动相为甲醇∶1%醋酸水溶液(90∶10);流速1.0 ml/min;柱温为室温,检测波长为215 nm。结果:测定福建省的枇杷叶样品12批次,标定出共有峰8个,其指纹图谱相似度均在0.9以上,指纹图谱整体面貌基本一致。精密度、稳定性、重现性试验中各共有峰相对峰面积的RSD均小于3%,符合有关规定。结论:该方法简便、快速、准确、重现性好,为枇杷叶的定性鉴别和药材内在质量评价提供了科学依据。
Object ： To establish the fingerprint spectrum of Loquat Leaf of Fujian authentic medicinal herbs by HPCE. Methods ： The HPLC fingerprints of Loquat Leaf were ohtained from instrument HP1100. The HPLC separation was performed on a Eclipse XDB- C18 （4. 6 mm× 150 mm, 5μm） analytical column diluted with methanol-1% glacial acetic acid （90： 10） at the flow rate of 1.0 ml/ min. The temperature of column was room temperature. The UV detection wavelength was 215 urn. Results： Detected 12 batches samples of different species Lopuat Leaf Fingerprint by the method of HPLC. Eight peaks in the chromatogram were common. All the similarity were above 0. 9, the fingerprints integer feature were coincident essentially. Conclusion： The method is convenient, quick and exact, and its reproducibility is good, It will be a scientific reference for qualitation identification and quality evaluation of Loquat Leaf.
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1234-1237,共4页
Journal of Chinese Medicinal Materials
基金
福建省重大科技攻关项目"创新药物与中药现代化"(2002y010)
