摘要
用离子交换和亲和层析色谱法从白眉蝮蛇毒中提取出了一种高纯度的具有激肽原酶活性的蛋白水解酶,称为蛇毒激肽原酶,该酶不需活化即可水解激肽原,释放激肽。粗毒经提纯,比活可达800U/mg以上,远远高于哺乳动物来源的激肽原酶,纯度可达95%以上。对其急性毒性及长期毒性进行研究,最大耐受剂量达人体最大给药剂量的15万倍,长期以人体给药剂量50倍给药,实验组动物的体质量、生存状态、肝功能、肾功能、血常规等无明显变化,证明蛇毒类激肽原酶毒性极低,可开发新型药物。
A novel serine protease with high purity was extracted from the venom of Agkistrodon halys Pallas using monoclonal antibody affinity claromatograpy.This proteas releases bradykinin and has arginine esterase activity without being activated.After purification,its hydrolytic activity exceeded 800 U/mg,far higher than its counterparts from mammalian sources.The purity of the kininogenase could exceed 95%.The acute toxicity and the long-term toxicity of this kallidrein was studied for its potential clinical application.The maximum tolerance dose in adult was 150000 times greater than the maximum applied dose,and long-term administration at the dose 50 times of allowed clinical dose did not obviously after the animals'body weight,survival condition,liver function,renal function,or blood routines,suggesting the extremely low toxicity of the kallidrein.
作者
张颖
马骉
林永齐
罗云波
ZHANG Ying;MA Biao;LIN Yong-qi;LUO Yun-bo(Graduate School of the Chinese Academy of Sciences,Beijing 100009,China;Beijing Saisheng Pharmaceutical Company,Beijing100176,China;College of Life Science,Jilin University,Changchun 130023,China;College of Food Science and NutritionalEngineering,China Agricultural University,Beijing 100094,China)
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第11期1756-1758,共3页
Journal of Southern Medical University
关键词
蝮蛇
激肽原酶
急性毒
长期毒性
Agkistrodon hlays Pallas
kallidrein
the acute toxity
long-term toxicity
