摘要
本次研究为了解决人血丙种球蛋白制品因纤溶酶原及纤格酶引起的IgG裂解问题，采用了L-赖氨酸与Sepharose4B偶联后吸附制品中纤溶酶原及纤溶酶。结果表明，人血丙种球蛋白中纤港酶原及纤溶酶被吸附。被吸附过的制品与未被吸附过的制品相比较其裂解程度显著降低。因此，在人血丙种球蛋白生产工艺中，加入L-赖氨酸-Sepharose4B吸附纤溶酶原和纤溶酶，可明显提高产品稳定性，解决人血丙种球蛋白裂解的问题，可提高产品的稳定性。
To increase stability and to solve the problem of splitting of human secrum γ -globulin products, we were used to adsorb fibrinolysin and profibrinoly8in with L - Lysine -Sepharose 4B. The Result shows that fibrinolysin and profibrinolysin in the human secrum γ -globulin products are adsorpted. Compared with the unadsorbed products, the splitting degree ofthe adsorbed products was decreased obviosly. In manufacture production of human secrum γ -globulin products, adsorbing fibrinolysin and profibrinolysin with L - Lysine - sepharose 4B mayincrease stability of human secrum γ - globulin and solve splitting problem of the product. It is aeffective method to improv the production quality.
出处
《药学实践杂志》
CAS
1997年第2期90-92,共3页
Journal of Pharmaceutical Practice
