摘要
目的研究重复感染致病毒性心肌炎小鼠血清转化生长因子-β1(TGF-β1)与心肌基质金属蛋白酶抑制剂-1(TIMP-1)及胶原表达相关性。方法连续4次经腹腔注射接种柯萨奇病毒B3m(CVB3m)感染小鼠,分别于末次感染后10、20、30、40、50、60d处死实验动物,ELISA法检查血清中TGF-β1水平;Western Blot法测定心肌中TIMP-1;氯胺T氧化法检测心脏胶原蛋白。结果血清中TGF-β1在末次感染后10d即见增加(37.4±12.6) ng/L;30d时实验组[(54.5±15.4)ng/L]明显高于对照组[(37.4±16.8)ng/L],P<0.05;60d时实验组[(123.6±28.5)ng/L]仍明显高于对照组[(38.8±13.5)ng/L],P<0.05。心肌中TIMP-1则在末次感染后40d升高(484±52);60d时实验组(483±47)仍显著高于对照组(417±48),P<0.05。心肌中胶原蛋白水平在末次感染后30d升高(11.84±1.94)g/kg;60d时实验组[(18.56±3.51)g/kg]仍明显高于对照组[(9.88±0.84)g/kg],P<0.05。结论CVB3m重复感染可导致血清中TGF-β1持续升高,增加的TGF-β1与TIMP-1的高表达有关,TGF-β1和TIMP-1都与病毒重复感染导致的心脏胶原异常增殖有密切关系。
Objective To investigate the relationship between TGF-β1 （transforming growth factor-β1 ） in serum and TIMP-1 （tissue inhibitors of metalloproteinase-1）, collagen in myocardium in myocarditis caused by repetitive CVB3m infection. Methods 4-week-old mice were infected with CVB3m 4 times successively followed by the sacrifice on the 10th, 20th, 30th, 40th, 50th and 60th day respectively after the final infection. Western Blot was used to determine the content of TIMP-1, while chloramine T oxidation was used detect the content of collagen in myocardium, ELISA for measuring T TGF-β1 level in serum. Results TGF-β1 began to elevate to（37.4 ± 12.6）ng/L 10 days after the final infection,dramatically higher than that of controls on the 30th day [（54.5 ± 15.4） vs （37.4 ± 16.8）ng/L] （P 〈 0.05） and on the 60th day[ （123.6 ± 28.5） vs （38.8 ±13.5 ）ng/L,P 〈 0.05]; TIMP-1 rose to（484 ± 52）on the 40th d,still higher than that of controlson the 60th day[（483 ± 47） vs （417 ± 48）P 〈 0.05] ; collagen in myocardium started to elevate on the 30th day to be （11.84 ± 1.94）g/kg, higher than that of controls on the 60th day [（18.56 ± 3.51）, （9.88 ＋ 0.84）g/kg, P 〈 0.05]. Conclusions Repetitive CVB3m infection can cause continuous increase of TGF-β1 in serum. Positively correlated with the expression of TIMP-1, TGF-β1 is closely correlated with ventricular remodeling caused by repetitive CVB3m infection.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2007年第6期634-635,共2页
Chinese Jouranl of Endemiology
