摘要
目的研究培本方对哮喘大鼠内源性糖皮质激素(GC)各作用途径的调节作用。方法采用OVA(卵蛋白)致敏、激发复制大鼠慢性哮喘模型,以阿斯美胶囊为对照药,通过放免法检测血清CORT(皮质酮)、血浆ACTH(促肾上腺皮质激素)和下丘脑CRH(促肾上腺皮质激素释放激素)的含量及免疫组化法检测肺组织GCR(糖皮质激素受体)的表达,探讨培本方及其拆方对大鼠哮喘模型的部分作用机制。结果模型对照组较正常对照组CORT及ACTH含量均显著降低、GCR蛋白表达亦明显降低、CRH含量无明显改变;培本方全方组及拆方Ⅱ组改善CORT,ACTH,GCR的影响作用更为显著。结论调节内源性GC的作用途径可能是培本方抗哮喘作用的机制之一。
Objective To study the adjustment of Peibenfang on the functional route of endogenous GC in asthma rats. Methods Asthma rats model was established by OVA through sensitizing and challenging. Asimei Capsule was used as positive control medicine. By investigating CORT in blood serum, ACTH in blood plasma, CRH in hypothalamus and GCR in the lung issue, the effects of Peibenfang on asthma were observed. Results The levels of CORT, ACTH and GCR in model group were lower than those in normal group ; in Peibenfang group and Peibenfang compatibility group （ part Ⅱ ） , these indexes increased remarkably. Conclusion One of the mechanisms of Peibenfang against asthma is to adjust the functional route of endogenous GC in asthma.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第11期2765-2767,共3页
Lishizhen Medicine and Materia Medica Research
