摘要
目的研究巫溪不同产地的款冬所含的芦丁的差异。方法HPLC测定款冬芦丁含量。流动相：甲醇．水（0．025ml／L）磷酸（40：60）；检测波长360nm．结果标准曲线为Y=6934．5X-3365（r=0．9999），芦丁在0．04008～0．4008μg范围呈良好的线性关系；平均回收率为99．40％，RSD为2．14％。结论该方法简便、快捷、准确；巫溪不同产地的款冬花的芦丁含量接近，适合在当地大规模栽培。
Objective To determine rutin in buds of Tussilago farfara L. from different areas. Methods The content of rutin was determined by HPLC with methanol - water（0.025 tool · ml^-1 ） phosphorous acid （40： 60） as mobile phase, detective wavelength was 360 nm. Results The standard curve for rutin was Y = 6 934.5X - 3 365 （ r = 0.999 9）, linear range was 0.040 08 - 0.400 8 pug, the average extraction recovery was 99.40%. Conclusion This method is simple, sensitive and rapid, and the contents of rutin in T. farfara from different areas are similar. Tussilago farfara L. is suitable to be planted in Chongqing Wuxi county.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第11期2722-2723,共2页
Lishizhen Medicine and Materia Medica Research
关键词
款冬花
巫溪县
高效液相色谱
芦丁
Tussilago farfara L.
Chongqing Wuxi county
HPLC
Rutin
