摘要
本文报道了红豆杉单细胞平板培养的过程．研究了细胞悬浮培养时间、培养方式和接种密度对红豆杉单细胞平板培养植板率的影响．结果表明，以悬浮培养１０－１４ｄ的单细胞为材料，接种密度为３－５×１０３／ｍｌ时。
Factors such as cell suspension culture time,the ways of plating culture and cell density,which affects the plating efficiency were investigated in this paper.The result showed that plating at density of 3-5×10 3cells/ml and with monocells seperated from 10-14 day cell,high plating efficiency could be botained either by conditional plate culture or by nursing plate culture.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
1997年第1期107-109,共3页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省教委自然科学基金
