摘要
探讨芍药苷(paeoniflorin,Pae)对佐剂性关节炎(adjuvant arthritis,AA)大鼠血清抗体水平和脾脏淋巴细胞cAMP水平的影响。大鼠足跖皮内注射弗氏完全佐剂(FCA)诱发大鼠AA模型,聚乙二醇(PEG)6000法检测血清循环免疫复合物(circulating immune complexes,CIC);酶联免疫吸附(ELISA)法检测血清中抗C II抗体(anti-C IIantibody)水平和抗结核杆菌抗体(anti-TB antibody)水平;放射免疫法(RIA)检测芍药苷体外给药对脾细胞中cAMP水平的影响。大鼠免疫后第17天开始分别灌胃给予芍药苷(25、50及100 mg.kg-1)和雷公藤多苷(40 mg.kg-1)对照药,连续给药7 d。芍药苷(50及100 mg.kg-1)可明显降低AA大鼠血清中升高的CIC水平、抗C II抗体水平和抗结核杆菌抗体水平,芍药苷组对AA模型组的抑制率(inhibition ratio,IR)与剂量呈正相关;芍药苷体外给药(12.5、62.5及312.5 mg.L-1)使AA大鼠脾细胞升高的cAMP水平降低。芍药苷治疗后,明显降低AA大鼠的血清抗体水平及大鼠脾细胞升高的cAMP水平。
This study is to investigate the effects of paeoniflorin （Pae） on the levels of related serum antibodies and cAMP of splenocytes in rats with adjuvant arthritis. Complete Freund＇ s adjuvant was used to induce AA in rats. The level of circulating immune complexes in serum was determined by PEG6000 assay, and the levels of anti-C II antibody and anti-TB antibody in serum were measured by enzyme-linked immunosorbent assay （ELISA） , the level of cAMP in splenocytes was measured by radioimmunoassay, separately. Pae （25, 50, and 100 mg·kg^-1） and GTW （40 mg·kg^-1） were given by intragastric administration for 7 days from the 17th day after immunization. Pae （50 and 100 mg ·kg^-1 ） reduced the levels of circulating immune complexes, anti-C II antibody and anti-TB antibody in serum in rats with adjuvant arthritis. The inhibition ratios of Pae groups to AA model group were dosage-dependent; Pae （ 12.5, 62.5, and 312.5 mg ·L^-1 ） decreased the elevated levels of cAMP in splenocytes in vitro. Pae （ig） decreased the levels of related serum antibodies and elevated the level of cAMP in rats with adjuvant arthritis.
出处
《药学学报》
CAS
CSCD
北大核心
2007年第11期1147-1151,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(30572356)
安徽省自然科学基金资助项目(050430903)
安徽省高校首批科技创新团队基金
高校博士点科研基金(20060366003).
