摘要
采用体外细胞培养技术,观察了钙离子阻断剂3、6-[=甲氨基]-=苯骈碘杂六环枸橼酸盐(I-65)单独和与柔红霉素(DNR)合用对L_(1210)细胞增殖的影响。结果:单独应用I-65时对细胞的增殖和集落形成抑制作用极小;与DNR合用,可显著增强DNR对细胞增殖的抑制作用。其ID_(50)为单用DNR的1/11。联合用药时细胞集落形成率较单用DNR(0.15μg/ml)下降14倍,证实I-65能显著增强DNR的抗癌活性,为广泛应用DNR,减轻心脏毒性,防止耐药提供新手段,为临床提供了一种新的抗癌药物增效剂。
3'6-dimethamidodibenzopyriodonium citrate (I-65) a new calcium cannel blocker for enhancing the cytotoxicity of daunomycin (DNR) in cultured L_(1210) leukemia cells in vitro. The ID_(50) of DNR to the cells is about 1.61vg/ml when the cells are treated with DNR alone. But when the cells are treated with DNR plus I-65 (5μg/ml), the ID_(50) of DNR is decreased to 1/11. In colony test. the survival ratio of the cells treated with DNR plus I-65 is decreased 14 times to the cells treated with DNR alone. So we suggest that I-65 can enhancing antitumor activity of DNR in vitro and may be used clinically as an antitumor drug enhancing DNR.
出处
《西北民族学院学报（自然科学版）》
1997年第1期42-44,共3页
Journal of Northwest Minorities University(Natural Science )
