摘要
目的:探讨上皮膜蛋白1基因(epithelial membrane protein 1,EMP1)在人脑胶质瘤中的表达及其与肿瘤恶性程度的相互关系。方法:肿瘤组织标本均取自上海长征医院神经外科2005至2006年部分手术病例,正常脑组织来源于捐献,分别采用逆转录酶聚合酶链反应(RT-PCR)和免疫组化染色方法检测33例星形细胞瘤、3例少枝胶质细胞瘤、2例室管膜瘤以及7例正常脑组织标本中EMP1 mRNA及蛋白的表达。结果:EMP1无论在基因还是在蛋白表达水平上,在胶质瘤和正常脑组织中均有不同程度表达,但在星形细胞瘤中表达的含量明显高于正常脑组织,且在星形细胞瘤中含量随着其病理分级的增高而增高,在低级别(WHOⅠ～Ⅱ级)与高级别(Ⅲ～Ⅳ级)之间存在显著差异(蛋白P<0.05;基因P<0.01)。结论:基因EMP1在人脑胶质瘤中显著高表达,并且与星形细胞瘤的恶性程度密切相关。
Objective： To investigate the expression of epithelial membrane protein 1 （ EMP1 ） in human glioma and its relationship with malignancy degree of tumors. Methods： The tumor specimens, including 33 astrocytomas, 3 oligodendrogliomas, and 2 ependymomas, were obtained during operation in the Department of Neurosurgery of Changzheng Hospital between 2005 and 2006 ; seven normal brain tissue samples came from donation. The expression of EMP1 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemical staining. Results： The expression of EMP1 mRNA and protein was detected in the gliomas and the normal brain tissues, with the expression in the astrocytoma specimens significantly higher than that in the normal brain tissues （P 〈 0.01 ） ; besides, the expression in astrocytomas increased with the pathological grades, with significant difference found between the WHO Ⅰ -Ⅱ and WHO Ⅲ-Ⅳ grades （P〈0.05 for protein, P〈0.01 for mRNA）. Conclusion： EMP1 is highly expressed in human glioma and the expression is closely related to the malignant degree of astrocytoma.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2007年第5期466-470,共5页
Chinese Journal of Cancer Biotherapy
基金
国家高科技研究发展(863)计划项目(No.2005AA001070)
Supported by the National High Technology Research and Development Program of China(No.2005AA001070)
