摘要
目的:探讨血管紧张素转换酶(angiotensin converting enzyme,ACE)在子痫前期发病机制中的作用。方法:原位杂交法检测30例子痫前期产妇(子痫前期轻度15例,重度15例)和12例正常产妇胎盘组织ACE mRNA的表达及分布;紫外分光光度法测定胎盘局部以及外周静脉血ACE活性。结果:ACE mRNA在各组产妇胎盘绒毛血管内皮细胞、滋养细胞中均可见表达。子痫前期组(3.12±0.94)明显高于对照组(1.65±0.67)(P<0.05);子痫前期重度组和子痫前期轻度组比较,差异有统计学意义(P<0.05)。子痫前期组胎盘组织及外周静脉血ACE活性明显高于对照组(P<0.05),子痫前期重度组和子痫前期轻度组比较,差异有统计学意义(P<0.05)。胎盘局部ACE活性与外周静脉血ACE活性呈正相关(r=0.781,P<0.05)。结论:胎盘局部ACE的表达及活性增高与子痫前期发病及病情发展密切相关。
Objective To investigate the effect of angiotensin converting enzyme （ACE） in the pathogenesis of preeclampsia. Methods A cross-sectional study was conducted with 42 pregnant women in the following categories： 30 cases of preeclampsia （mild preeclampsia, n = 15; severe preeclampsia, n = 15 ） , and normal pregnancy （ control group, n = 12 ）. The expression and localization of ACE mRNA in the placenta of the 3 groups were respectively examined by in situ hybridization. Ultraviolet radiation colorimetry was used to detect the activity of ACE in the placenta tissue homogenate and the mothers＇ serum in the 3 groups. Results The expression of ACE mRNA was found in the endothelial cells of villus and trophoblasts in the placenta. The positive index of ACE mRNA in the placenta of preeclampsia （3. 12 ± 0.94 ） was higher than that in the normal pregnancies（ 1.65± 0.67 ） （P 〈 0.05）, and there was significant difference between severe preeclampsia and mild preeclampsia （ P 〈 0.05 ）. The levels of ACE activity in the placenta tissue homogenate and the maternal serum of preeclampsia were higher than those in the normal pregnancies （P 〈 0.05）, and there was significant difference between severe preeclampsia and mild preeclampsia （P 〈0.05）. The placenta tissue homogenate ACE activity was correlated with ACE activity of the maternal serum （ r = 0.781 , P 〈 0.05 ）. Conclusion The expression and activity of local ACE in the placenta tissue may play an important role in preeclampsia and contribute to the development of preeclampsia.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2007年第3期498-502,共5页
Journal of Central South University ：Medical Science
关键词
血管紧张素转换酶
子痫前期
胎盘
原位杂交
angiotenson converting enzyme
preeclampsia
placenta
in situ hybridization
