摘要
目的:通过观察去卵巢小鼠股骨内OPG、RANKL和ApoE的变化来探讨绝经后女性骨质疏松的发病机制。方法:采用双侧卵巢切除术制备骨质疏松的动物模型,1周后雌激素替代组开始腹腔注射苯甲酸雌二醇(EB)。6周后应用免疫组化法观察小鼠股骨内OPG、RANKL、ApoE的变化。结果:去卵巢组股骨内OPG、RANKL和ApoE的表达与对照组相比明显下降,而补充雌激素后OPG、RANKL和ApoE的表达明显上升。结论:绝经后女性骨质疏松与雌激素具有相关性,雌激素可通过增加OPG、RANKL和ApoE的表达抗骨质疏松。
Objective： To discuss the mechanism of osteoporosis in post -menopause women through investigating influence of estrogen on OPG, RANKL and APOE in ovariectomized mice. Methods： All mice were divided into three groups -control groups, ovariectomized groups and estrogen- complemented groups. One week later, estrogen -complemented mice were infused with estradiol benzoate twice each week. Control groups and ovariectomized groups were infused with the same does of olive oil. Six weeks later, immunohistochemistry and image - analysis were used to test the changes of OPG, RANKL and APOE in each group. Results： The level of OPG, RANKL and APOE were increased in estrogen - complemented groups, but decreased in ovariectomized groups. Conclusion： There is close relationship between estrogen and osteoporosis in post- menopause women. Estradiol benzoate improves the level of OPG, RANKL and ApoE to resist osteoporosis.
出处
《中国妇幼保健》
CAS
北大核心
2007年第32期4601-4603,共3页
Maternal and Child Health Care of China
