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肾癌G250 MN／CAIX重组质粒DNA免疫效应的初步研究 被引量：1

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摘要 [目的]观察PCDNA3.0-G250重组质粒在小鼠中诱导产生DNA免疫应答的特点及抑瘤效应。[方法] PCDNA3.0-G250质粒每隔2周肌注射Balb/c小鼠共3次,对照组同法肌注PCDNA3.0质粒。每次免疫10 d后眶后取血,间接免疫荧光法检测血清抗体。6周后取脾细胞观察体外细胞毒效应。同法免疫Balb/c小鼠,第3次免疫时皮下种植sp2/0- PCDNA3.0-G250细胞,观察肿瘤大小及小鼠生存期。[结果]DNA免疫后可检测到特异性抗体,抗体滴度随免疫次数增加逐渐升高,差异有统计学意义(P<0.01)。体外CTL杀伤率与对照组差别有统计学意义。体内实验发现治疗组与对照组抑瘤效应无明显差异(P>0.05)。[结论]G250重组质粒DNA免疫能在小鼠巾诱导产生特异性细胞免疫及体液免疫,无明显体内抑瘤效应。
作者 李晓飞 刘雷 吴荣佩 张亚东 李乐军 徐秀章 曹开源
机构地区 中山大学附属第一医院泌尿外科 珠海市人民医院 中山大学检验医学系
出处 《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2007年第B06期7-9,共3页 Journal of Sun Yat-Sen University:Medical Sciences
基金 广东省科技攻关资助项目(2003c31201) 广州市科技攻关资助项目(2003Z22E4011)
关键词 肾肿瘤 癌 肾细胞癌 G250/MN/CAIX抗原 DNA免疫
分类号 R699 [医药卫生—泌尿科学]
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参考文献10

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二级参考文献6

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共引文献12

同被引文献11

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  • 10张亚东,吴荣佩,刘雷,李乐,曹开源,李晓飞.肾癌G250抗原基因在sp2/0细胞中的表达及其意义[J].中山大学学报（医学科学版）,2008,29(1):16-19. 被引量：1

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中山大学学报（医学科学版）
2007年 第B06期

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