摘要
目的了解染色体１７ｐ１３．３丢失及ｐ５３蛋白异常表达在胃癌发生发展中的作用。方法应用ＰＣＲ－ＲＦＬＰ法及免疫组化技术检测了５１例胃癌手术标本中１７ｐ１３．３杂合性丢失及ｐ５３蛋白异常表达。结果发现在３１例信息个体中１７ｐ１３．３杂合丢失１６例，占５１．６％（１６／３１）。ｐ５３蛋白染色阳性１９例，占３７．３％（１９／５１）。１７ｐ１３．３杂合丢失与ｐ５３蛋白表达间无显著相关。１７ｐ１３．３杂合丢失率和ｐ５３蛋白阳性率在肠型及胃型胃癌差异无显著性（Ｐ＞０．０５），与临床病理参数无显著相关。１例早期胃癌检出１７ｐ１３．３杂合丢失。结论染色体１７ｐ１３．３杂合丢失、ｐ５３蛋白异常表达是胃癌的常见改变；１７ｐ１３．３杂合丢失可出现在胃癌的早期。
Objective To evaluate the role of17p13.3l oss and abnormal expression of p53 in the development and progress of gastric carcinoma.Methods Theloss of heterozygosity(LOH) at 17p13 3 and abnormal expression of p53 gene were examined with PCR RFLP and immunohistochemical methods in 51 surgical specimens of gastric carcinoma.ResultsL OH at 17p13.3 was detected in 16/31 cases(51.6%)and positive staining for p53 protein was found in 19/51 cases (37.3%).No significant correlation was found between LOH at 17p13.3 and abnormal expression of p53 or between these two events in any clinicopathological parameters.LOH was found in one cases of early gastric cancer.Conclusions These results suggest that LOH at 17p and abnormal expression of p53 protein are frequently found in gastric carcinoma and may play an important role in the carcinogenesis and progress of both intestinal and gastric types of gastric cancer.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1997年第3期134-136,共3页
Chinese Journal of Pathology
基金
国家自然科学基金
