摘要
对在６个月内从１２１名产科新生儿中的３０名检出的３１株铜绿假单胞菌进行ＲＡＰＤ指纹图谱分析和血清学分型。２４株为Ｏ：６血清型，６株属于另外３个血清型，１株未能分型。ＲＡＰＤ分型率为１００％。３１个菌株分成５个ＲＡＰＤ谱型。２５株为Ｒ：１型，其它６株为另外的４个谱型。血清型不同的新生儿菌株的ＲＡＰＤ谱型亦不相同。２株Ｏ：１型的菌株，其ＲＡＰＤ谱型却不同。同期分别来自４个其他科室的５名患者的５株Ｏ：６型ＰＡ菌的ＲＡＰＤ谱型各自不同，与新生儿Ｏ：６型的ＲＡＰＤ谱型也不同。结果表明，铜绿假单胞菌在产科新生儿的暴发流行，Ｏ：６／Ｒ：１型为暴发流行菌株；ＲＡＰＤ分型率高，分辨力强，快速简便，颇具医院感染分子流行病学研究的应用价值。
Randomly amplified polymorphic DNA(RAPD) fingerprint and serotyping were used to type 31 strains of Pseudomonas aeruginosa isolated from 30 out of 121 new borns in an obstetrical ward during a period of 6 months. Twenty-four strians were serotype O: 6, 6 strains other 3 serotypes, 1 isolate not typeable by serotyping. The typeability of RAPD was 100%. Five different RAPD profiles were obtained from 31 new born strains, and 25 strains had the same profiles. The strains with the different serotype were also different in RAPD profiles. Two O: 1 strains had different RAPD profiles. The profiles of 5 O: 6 strains from 5 patients in 4 different wards were distinguishable from each other and from newborn O: 6 strains. The results showed that an outbreak of Pseudomonas aeruginosa occurred in the newborns, type O: 6/R: 1 was the outbreak strain, and RAPD is a very useful tool with high typeability and discrimina-tory power for molecular epidemiological study of nosocomial infections.
出处
《中华医院感染学杂志》
CAS
CSCD
1997年第2期69-71,共3页
Chinese Journal of Nosocomiology
基金
福建省卫生厅科研基金
关键词
铜绿假单胞菌
DNA
分子流行病学
新生儿感染
Pseudomonas aeruginosa Molecular epidemiology Fingerprint Randomly amplified polymorphic DNA New born
