摘要
目的探讨中国汉族急性冠脉综合征（ACS）患者尿酸与内皮型一氧化氮合酶（eNOS）基因多态性的关系。方法采用聚合酶链反应/限制性片段长度多态性方法分析58例ACS患者（ACS组）和43例对照组患者的eNOS基因Glu298→Asp变异体。结果与对照组比较,ACS组eNOS基因Glu/Glu、Glu/Asp、Asp/Asp基因型频率差异无统计学意义（43.1%、36.2%、20.7%比48.8%、34.9%、16.3%,χ^2=0.446,P=0.800）。与eNOS基因Glu298等位基因携带者比较,ACS的危险性在Asp/Asp携带者中并不增高相对比值比（OR）1.34,95%可信区间（CI）0.479~3.755,P=0.575。eNOS基因Glu298→Asp变异体与Duke积分无显著性关系Glu/Glu（48.33±19.61）分,Glu/Asp（38.19±15.12）分,Asp/Asp（46.73±19.90）分,P=0.248;但Glu298→Asp基因型与ACS组患者血清尿酸存在显著的关联Glu/Glu（298.92±87.27）μmol/L,Glu/Asp（370.80±95.80）μmol/L,Asp/Asp（346.16±93.71）μmol/L,P=0.017。结论在中国汉族人群中,eNOS基因Glu298→Asp多态性与ACS患者不相关,而与ACS组患者血清尿酸存在显著的关联。
Objective To evaluate the association of elevation in serum uric acid with the development of coronary artery disease, and to determine the relationship between uric acid and Glu298→Asp polymorphism of the endothelial nitric oxide synthase （eNOS） gene in acute coronary syndrome （ACS） in the Chinese Han Nationality. Methods The Glu298→Asp variant of the eNOS gene was detected by polymerase chain reaction/restriction fragment length polymorphism analysis in 58 patients with ACS and 43 healthy controls. The severity of ACS was expressed by the number of affected vessels and by the Duke scoring system. Results The frequencies of the eNOS Glu/Glu, Glu/Asp, and Asp/Asp genotypes in the ACS group were not significantly different from those of controls （43.1%, 36.2%, 20.7% vs. 48.8%, 34.9%, 16.3%, respectively; χ2=0.446, P=0.800）. In comparison with subjects who had Glu298 allele in the eNOS gene, the risk of ACS was not increased among Asp/Asp carriers （odds ratio 1.34,95% confidence interval 0.479 to 3.755, P=0.575）. There was no significant association between the eNOS Glu298→Asp variant and the Duke score （46.73±19.90） score for Asp/Asp vs. （48.33±19.61） score and （38.19±15.12） score for Glu/Glu and Glu/Asp, respectively, P=0.248, but there was a significant association between the eNOS Glu298→Asp variant and the serum uric acid level in ACS group （298.92±87.27） μmol/L for Glu/Glu vs. （380.80±95.80） μmol/L and （346.16±93.71） μmol/L for Glu/Asp and Asp/Asp,respectively, P=0.017. Conclusion Glu298→Asp polymorphism of the eNOS gene appears to have no association with ACS in the Chinese Han Nationality, but a significant association between the eNOS Glu298→Asp variant and the serum uric acid level is found in patients with ACS.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2007年第11期652-656,共5页
Chinese Critical Care Medicine
基金
国家“十五”重大科技（863）项目（2002DA711A08-21）
甘肃省教育厅科研项目（043-03）
河北省科学技术研究与发展指导计划（04276139）
关键词
急性冠脉综合征
一氧化氮
内皮型一氧化氮合酶
遗传
尿酸
基因表达
acute coronary syndrome
nitric oxide
endothelial nitric oxide synthase
genetics
uric acid
gene expression
