摘要
目的优化制备更昔洛韦脂质体的处方。方法采用逆相蒸发法制备脂质体,在单因素实验的基础上,以包封率和泄漏率为指标综合评分,采用正交设计实验的方法优化制备更昔洛韦脂质体的处方。结果更昔洛韦脂质体的最佳制备处方为:药物∶(磷脂+胆固醇)为1∶12(质量比),胆固醇与卵磷脂质量比为1∶5,吐温-80∶(磷脂+胆固醇)比为1∶15(质量比),有机相与水相体积比为1∶2.5,在60℃时采用逆向蒸发法制备,制得的更昔洛韦脂质体包封率达43.24%,泄露率为1.02%,粒径为332.3nm。结论按最优制备处方制得的更昔洛韦脂质体的包封率较高,泄漏率较低。
Objective To optimize the formulation of ganciclovir liposome. Methods Orthogonal design was adopted to screen the for-mulation of ganciclovir liposome on the basis of the score of the entrapment efficiency and leaking percentage. Results The optimization conditions are drug： lipid=1：12, cholesterol： lecithin= 1：5, tween-80：lipid=1：15, chloroform：water=1： 2.5. The entrapment efficiency and leaking percentage of liposome, which was prepared on the optimization conditions, are 43.24% and 1.02%, respectively,and the particle size of the liposome was 332.3nm. Conclusion The entrapment efficiency of the optimized ganciclovir liposome and the leaking percentage are desirable.
出处
《西北药学杂志》
CAS
2007年第6期317-319,共3页
Northwest Pharmaceutical Journal
关键词
脂质体
更昔洛韦
包封率
正交实验
liposome
ganciclovir
entrapment efficiency
orthogonal design
