摘要
目的系统研究各种胃粘膜病变中ｐ５３抑癌基因的变异和意义．方法内镜活检组织１５４例，其中浅表性胃炎３０例，萎缩性胃炎３３例，萎缩性胃炎伴肠上皮化生３１例，萎缩性胃炎伴异型增生３０例，胃腺癌３０例．用免疫组化法检测Ｐ５３蛋白表达；用单链构象多态性分析及ＤＮＡ测序检测ｐ５３第５～８外显子点突变．结果胃良性病变粘膜未见Ｐ５３蛋白表达．胃癌中Ｐ５３蛋白阳性表达率为３３３％（１０／３０例）．单链构象多态性分析ｐ５３点突变的检出率，在胃癌为５４５％（６／１１例），异型增生为２０％（３／１５例），肠化生为６７％（１／１５例），测序证实１例胃癌在第５外显子存在点错义突变和碱基缺失，２例异型增生在第６外显子存在点错义突变．结论ｐ５３抑癌基因变异与胃粘膜癌变有关．
AIM To study the alteration of p53 in a variety of gastric mucosal lesions. METHODS Immunohistochemical staining was used to examine the overexpression of p53 protein in 154 cases of human gastric mucosal lesions from endoscopic biopsies. We also used PCR-SSCP analysis and DNA sequence to detect p53 exon 5 to 8 mutation. RESULTS Positive nuclear staining for p53 protein was 33 3%(10/30 cases) in gastric carcinoma except non-malignant mucosal lesions, and p53 mutation of exon 5 to 8 was determined in 6 7% (1/15 cases) of intestinal metaplasia, 20% (3/15 cases) of dysplasia, and 54 5% (6/11 cases) of gastric carcinoma. We confirmed p53 misense mutation (G→T transversion) and delete C of exon 5 in gastric carcinoma and misense mutation (G→C transversion) of exon 6 in dysplasia by using DNA sequence analysis. CONCLUSION Alteration of p53 suppression gene may be closely associated with the malignant transformation of gastric mucosa.
基金
国家863基金
关键词
胃肿瘤
癌变
胃粘膜
抑癌基因
P53
P53 protein Gastric mucosa Gastritis Stomach neoplasms Gene, suppression, tumor
