摘要
目的评价虫草多糖脂质体的抗肝纤维化作用．方法采用小剂量虫草多糖脂质体（虫草多糖量为１５ｍｇ／１００ｇ，隔日１次）治疗ＣＣｌ４所致雄性Ｗｉｓｔａｒ大鼠肝纤维化（ｎ＝１２）１０周．运用地高辛自由引物法标记的１７ｋｂ胶原酶探针行点渍印迹杂交检测大鼠肝组织中的胶原酶ｍＲＮＡ含量．结果小剂量虫草多糖脂质体使大鼠肝组织中胶原酶ｍＲＮＡ含量增高非常显著（灰度数字转换值，７９１５±２０２３ｖｓ３１３０±６９２，Ｐ＜００１）．结论虫草多糖脂质体通过增加肝组织胶原酶ｍＲＮＡ的表达，促使Ⅰ，Ⅲ型胶原降解，可能是其抗纤维化的主要机制之一．
AIM To study the mechanism of cordyceps polysaccharides_liposomes (CSP_L) treatment on CCl 4 induced liver fibrosis in rats. METHODS Digoxin-labelling collagenase probe was used to detect the interstitial collagenase mRNA expression in CCl 4 induced liver fibrosis in CSP-L treated rats ( n =12) and compared with non_CSP-L treated rats ( n =6) dot blot. RESULTS The mRNA expression of interstitial collagenase in rats after treatment with CSP-L was significantly higher than that of non_treated rats (791 5±202 3 vs 313 0±69 2, P <0 01). CONCLUSION CSP_L could resolve the liver collagen Ⅰ,Ⅲ by stimulating collagenase activity.
关键词
肝纤维化
虫草多糖脂质体
胶原酶
MRNA
Liver cirrhosis Cordyceps polysacharid esliposome Collagenase RNA, messenger
