摘要
目的利用非抗凝猪血简易制备原卟啉二钠。方法提取非抗凝猪血中的血红素、经氯化血红素、原卟啉二甲酯、原卟啉等中间制备过程,最后制取原卟啉二钠。结果所制得的原卟啉二钠为紫黑色晶体,可溶于水和甲醇,不溶于氯仿、乙醚、丙酮,难溶于稀酸,纯度为91.4%。结论此方法可利用非抗凝动物血中的血红素制备原卟啉二钠。该方法制备条件要求不高,操作简便、原料易得、成本低廉,是一种较为理想的制备原卟啉二钠的方法。
Objective To study simple preparation of protoporphyrin disodium using whole blood of animals. Methods The non-coagnlant blood, directly taking from swine, was defibrinated and heated with glacial acetic acid to get haemin, after esterification of haemin, crude protoporphyrin dimethyl ester was obtained in toluene and then saponified by sodium hydroxide ,and more purifing protoporphyrin disodium was obtained. Results The protoporphyrin disodium obtained , the purity in 91.4%, was a dark brown red solids. It was soluble in water ,acidic solution and insoluble in ethyl ether. Conclusion It is an ideal approach that protoporphyrin disodium can be compounds directly by whole blood with its course of production simple and economic value high.
出处
《热带病与寄生虫学》
2006年第3期147-148,共2页
Journal of Tropical Diseases and Parasitology
关键词
非抗凝猪血
血红素
原卟啉二钠
制备
Whole blood of swine, Haemin, Protoporphyrin disodium, Preparation
