摘要
目的以不同强度训练大鼠停训28周为对象,应用免疫组织化学技术探讨长期停训对大鼠脑组织的影响。方法雄性SD大鼠(3月龄,由西安交通大学医学院动物管理中心提供)24只,体重248±24g,国家标准啮齿类动物干燥饲料喂养,自由饮食,温度22℃-27℃,湿度40%-60%。适应性喂养1周后进行实验。将大鼠随机分为安静对照组、有氧训练组和疲劳训练组,每组各8只。有氧训练组和疲劳训练组运动负荷参照Bedford跑台训练模型训练8周。8周后疲劳训练组和有氧训练组停训,与对照组同处于正常笼内生活状态,再饲养28周。各组大鼠依次用乌拉坦腹腔麻醉,开胸行左心室穿刺灌注,同时剪破右心耳放血。灌注过程中,先用加3‰肝素生理盐水灌注15 min左右,直至流出液体为粉红色时换用中性甲醛固定液灌注40min左右,pH=7.4,将灌注好的标本置于事先准备的冰盘上并迅速取材。结论:大鼠恢复28周后有氧、疲劳训练使海马CA1区的表达增强,未见显著性差异。显示该区在训练恢复后VEGF阳性表达基本恢复。海马CA3区,VEGF的表达下降趋势显著。
This paper studies the feature of the tissue-brain tissue of stopping training 28 weeks rats on the base. There are totally 24 male SD rats, which are provided by the Medical School of Xi＇an Jiaotong University. They are feed naturally by standard dry feed, their weights are 248 ±24g and they live under the temperature of 17℃-23℃, and momisture of 40%- 60%. They are randomly divided into three groups： sedentary group （8 rats）, aerobic training group （8 rats） and fatigue training group （8 rats）. After one week of natural feeding, Aerobic training group and fatigue training group received 3-day training, with the speed of 15rrdmin in 20min. Aerobic training group： 5 days per week, 8 weeks, accelerating speed of 3m/rain in 5min to 35m/min. After 60 min, to add 50 to the treadmill. Fatigue training group： 6 days per week, 8 weeks, accelerating speed of 3 m/min in 5 min to 20 m/min. After 60 min, to add 10o to the treadmill. All of rats are stopped training after 28 weeks and from then on taken to live freely in the cage. Later, all big mouse in the normal cage live 28 weeks. The result shows that after stopping training aerobic training promotes VEGF expression in CA1 area of hippocampus, Fatigue training restrain VEGF expression in CA3 area of hippocampus obviously.
出处
《北京体育大学学报》
CSSCI
北大核心
2007年第10期1370-1372,共3页
Journal of Beijing Sport University
