摘要
目的研究雷州半岛地区汉族人群血小板膜糖蛋白(GP)ⅠbKozak序列-5T/C和纤维蛋白原(Fib)β-148C/T基因多态性位点与脑梗死的关系。方法选择雷州半岛地区汉族人群健康体检者130例(对照组)和经头颅CT和(或)MRI证实的脑梗死患者148例(脑梗死组),用PCR-RFLP检测GPⅠbKozak序列-5T/C和Fibβ-148C/T基因多态性,分析两组频率分布特点及与脑梗死的关系。结果脑梗死组β-148C/T位点T等位基因频率为30.1%,对照组为21.9%,(P<0.05),脑梗死组GPⅠbKozak序列C等位基因频率为48.7%,对照组为39.6%,(P<0.05),两个多态性位点连锁不平衡系数D为-0.018(P>0.05)。结论Fibβ-148C/T位点T等位基因和GPⅠbKozak序列C等位基因是雷州半岛地区汉族人群脑梗死的遗传易感因素,但二者不存在连锁不平衡关系。
Objective To study the relationship between platelet membrane glycoprotein Ⅰ b Kozak sequence-ST/C gene,beta fibrinogen-148C/T gene polymorphism and cerebral infarction in Chinese Leizhou peninsula Hans. Methods A case-control study of 148 cases of cerebral infarction （CI group） confirmed by CT or MRI and 130 healthy controls（control group） was carried out. The platelet membrane glycoproteinⅠ b Kozak sequence-ST/C and beta fibrinogen -148C/T genotypes and gene frequencies were analyzed by polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP）. Results The frequencies of beta-Fib-148C/T gene T allele in CI group and control group were 30. 1% and 21.9%,respectively （ P 〈0.05）. Beta Fib-148C/T gene T allele carriers had 2. 023-fold increased risk for ischemic cerebrovascular events （ OR = 2. 023,95% confidence interval. 1. 157- 3. 537, P =0. 0134） over C/C genotype carriers. The frequencies of GP I b Kozak T/C C allele in CI group and control group were 48.7% and 39.6%,respectively （ P %0.05）. Homozygous C/C genotype carriers had 2. 541-fold increased risk for ischemic cerebrovascular events （ OR = 2. 541,95% confidence interval. 1. 124- 5. 744, P = 0. 0250） over T/T or T/C genotype carriers together. The coefficient of linkage disequilibrium for the two gene polymorphisms was - 0. 018 （ P 〉 0. 05）. Conclusions The glycoprotein I b Kozak-ST/C C/C genotype and beta Fib-148C/T gene T allele may be the genetic factors for susceptibility to cerebral infarction, but there is no disequilibrium linkage between the two gene polymorphisms.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2007年第11期745-748,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
