摘要
在实验室条件下培养7种(13株)杜氏藻(Dunaliella),在生长的平衡期收获.分别采用索氏提取法和气相色谱法进行了总脂含量和脂肪酸组成的测定.它们的总脂含量在6.64%~16.18%(m/m,干重,下同)之间,其中7株的总脂含量超过10%.杜氏藻的16碳和18碳脂肪酸含量丰富,有着较高的C16∶0、C18∶1(n-12)、C18∶2(n-6)和C18∶3(n-3)脂肪酸,其中8株的不饱和脂肪酸含量超过总脂肪酸的50%.杜氏藻的EPA和DHA的含量较低,但是有5株超过总脂肪酸的10%.
13 strains in 7 species of Dunaliella were cultured under defined conditions and harvested during their late log phase of the growth curves. Total lipid contents were determined by the Soxhlet procedure. Fatty acid compositions were performed on a GC. The results showed that the total lipid contents in 7 strains of Dunaliella were higher than 10% of their dry weight ( 11.61% -34. 49% ).The major fatty acids in most species (strains) were C16: 0, C 18: 1 ( n - 12 ) , C 18 : 2 ( n - 6 ) and C18:3 (n -3). High level of unsaturated fatty acids were found in 8 strains of Dunaliella (more than 50% of total fatty acid). EPA and DHA levels were lower in Dunaliella, however 5 in 13 strains showed that their EPA and DHA contents were higher than 10% of total fatty acid.
出处
《台湾海峡》
CAS
CSCD
北大核心
2007年第4期516-521,共6页
Journal of Oceanography In Taiwan Strait
基金
福建省重大专项前期研究项目(2005YZ1022)资助
关键词
杜氏藻
总脂含量
脂肪酸
Dunaliella
total lipid
fatty acid composition